FastQCFastQC Report
Fri 14 Jul 2023
SRR9047188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9047188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27185042
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7277402.6769868518135818Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT2443180.8987221722887166No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT2194800.8073557510045414No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1954440.7189394814986859No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1414430.5202971545896453No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA985690.36258542473467575No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA812990.29905784217659104No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG805100.2961555108136305No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA777610.28604333221188327No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC727290.2675331529743452Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA627910.2309762846789054No Hit
ATTGCACTCCGGATGTGCTGACCCCTGCGACTGTAGGCACCATCAATAGA605840.22285784954829205No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC601370.22121356295862998Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT595960.2192234979809853No Hit
TCTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAG558610.20548432479890966No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA538950.19825240659918789No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA509020.1872426755860815No Hit
TCGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG502470.18483326235067063No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAAT458050.16849339427174695No Hit
TCGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGA452580.16648125833316718No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG442960.16294254759657903No Hit
AGGAGCGGTAGTTCAGTCGGTTAGACTGTAGGCACCATCAATAGATCGGA441740.16249377139089946No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC404670.14885759602652077No Hit
TTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGC377180.1387454174247735No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA345270.1270073447008101No Hit
TGAAATTCCTTGTAGACTACGAGGTTGATCTGTAGGCACCATCAATAGAT333300.12260418799426537No Hit
TCGACCCCTAAGGCGAGGCCGAAACTGTAGGCACCATCAATAGATCGGAA329030.12103347127438685No Hit
ACGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGA328050.1206729789124475No Hit
AACGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAG327810.12058469506870727No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG314980.11586518792209334No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT314850.11581736750673403No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA308950.11364705634811967No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC308100.11333438440153965No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACATCAA307790.11322035110337515No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC297470.10942414582254462No Hit
AGGGGCTATAGCTCAGCTGGGAGACTGTAGGCACCATCAATAGATCGGAA290730.1069448412108394No Hit
CGGTGGTTCAAGTCCACTCAGGCCTACCACTGTAGGCACCATCAATAGAT289150.10636363923954946No Hit
TGTCGACCCCTAAGGCGAGGCCGAAACTGTAGGCACCATCAATAGATCGG281860.10368201748593951No Hit
TAAGATCCCATGTAGACCACATGGTTGATCTGTAGGCACCATCAATAGAT275140.10121006986121264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)