FastQCFastQC Report
Wed 13 Sept 2023
SRR9047189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9047189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147940080
Total Bases22.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA96418206.517381902186345Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG27550821.8622958700576613No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC23508391.5890480794656863No Hit
CTGTAGGCACCATCAATCCTGTAGGCACCATCAATAGATCGGAAGAGCAC14317750.9678073717413157No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA9058670.6123202042340385No Hit
CTGTAGGCACCATCAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC7506250.5073844762014459No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG7491680.5063996180075068No Hit
CTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGC7451710.5036978484802765No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA7054770.4768667152268675No Hit
TTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCA6823740.46125025753669996No Hit
TTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGC6318030.42706682327061063No Hit
AAATCCGGCCCCCGCAACCACTGTAGGCACCATCAATAGATCGGAAGAGC6007010.40604344677926363No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC5958440.40276036081635214No Hit
TCTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAG5840260.39477199147114156No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG5761420.38944280684450083Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAGA5027910.33986124652629635No Hit
TCGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGA4996680.33775025672556075No Hit
AGGAGCGGTAGTTCAGTCGGTTAGACTGTAGGCACCATCAATAGATCGGA4867390.3290109076593713No Hit
ACGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGA4770530.3224636623151752No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC4746660.32085017123148774No Hit
AATCCTCTATCACCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCA3982840.26921980845217874No Hit
ACCAGTGGGTCGGAGGTTCGACTGTAGGCACCATCAATAGATCGGAAGAG3956500.26743935788056894No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT3805260.25721630000470463No Hit
AACGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAG3783600.2557521937260004No Hit
AGACCGCCAGCTAAGGTCCCAACTGTAGGCACCATCAATAGATCGGAAGA3710910.25083871794580614No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3594710.24298418657067106No Hit
GGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAGAG3515170.23760768548996325No Hit
CGGTGGTTCAAGTCCACTCAGGCCTACCACTGTAGGCACCATCAATAGAT3381230.22855401997889957No Hit
AGGGGCTATAGCTCAGCTGGGAGACTGTAGGCACCATCAATAGATCGGAA3369930.22779019722038812No Hit
ACGAAGGGGTCGGAGGTTCGACTGTAGGCACCATCAATAGATCGGAAGAG3084640.2085060383906782No Hit
AATCCGGCCCCCGCAACCACTGTAGGCACCATCAATAGATCGGAAGAGCA2985170.20178237026774623No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA2932360.19821268178305704No Hit
TAGGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAG2889480.19531421099677654No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2847270.19246102881653168No Hit
AGTCCCGTCCGTTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA2737830.18506343919781576No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT2726140.18427325441489553No Hit
AGGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATAGATCGG2715940.1835837860842038No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT2678450.18104965199423984No Hit
AGGGTCGTTAGCTCAGTTGGTAGACTGTAGGCACCATCAATAGATCGGAA2672140.1806231279582923No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2501010.16905560683757911No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2475820.16735289044050808No Hit
AAGTCCACTCAGGCCTACCACTGTAGGCACCATCAATAGATCGGAAGAGC2456950.16607737402872838No Hit
CGGGGCTAAACCATGCACCGACTGTAGGCACCATCAATAGATCGGAAGAG2447250.16542170316522742No Hit
AAGGCTTGTAGCTCAGGTGGTTAGACTGTAGGCACCATCAATAGATCGGA2427630.1640954905526616No Hit
AAAATCCGGCCCCCGCAACCACTGTAGGCACCATCAATAGATCGGAAGAG2377180.16068532611311284No Hit
AGCTGATATAGCTCAGTTGGTAGACTGTAGGCACCATCAATAGATCGGAA2202690.14889068601287764No Hit
CGCGGGTTCGAGTCCCGTCCGTTCCGCCACTGTAGGCACCATCAATAGAT2159540.14597396459431414No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA2148220.14520878993711508No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGA2014850.13619365353864887No Hit
ACCGGAGAATGTTATCACGGGAGACACCTGTAGGCACCATCAATAGATCG1991250.13459841308724452No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT1871320.12649175260686624No Hit
AATCCTCTCTCGCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCA1870990.12646944627852033No Hit
TAGGCCGGGTGTGTAAGCGCAGCGACTGTAGGCACCATCAATAGATCGGA1854700.12536832479744503No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1816990.1228193198219171No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1783730.12057111230438702No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1761280.11905360602752141Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGAGCCGAAGGGAAACCGACTGTAGGCACCATCAATAGATCGGAAGA1706440.11534669982603768No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1688980.11416649227173598No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1649000.11146404679516193No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1627090.10998304178286236No Hit
CCTTCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAG1577520.10663236088556934No Hit
TCATCATGACCCTTTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1568630.10603144191891745No Hit
TCGACCCCTAAGGCGAGGCCGAAACTGTAGGCACCATCAATAGATCGGAA1568200.1060023760971334No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1538750.10401170527959698No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACATCAATAGATCGGA1534340.1037136116189744No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1491520.10081919652875679Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)