FastQCFastQC Report
Sat 30 Sept 2023
SRR9047195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9047195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66706669
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length25-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGATTAAGCCATGCATGTCTAAGTACGCA5254600.7877173420246784No Hit
TAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCT5157770.7732015520067417No Hit
TAAGATCCCATGTAGACCACATGGTTGAT5109810.7660118660699428No Hit
TGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGC4210350.631173773644731No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3859580.5785898258538438No Hit
TGATGAAGAACGCAGCTAGCTGCGAGAATT3556900.5332150523060895No Hit
AAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTT3123700.46827401919889No Hit
TGCCCGATCTCGTCTGATCTCGGAAGCTAAGC3004220.4503627665773568No Hit
TTGTCGATGAAGAACGCAGCTAGCTGCGAGAATT2683970.40235407347352337No Hit
TCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGC2621000.3929142377053785No Hit
AGCTAGGATGTTGGCTTAGAAGCAGCCATC2584210.3873990470128257No Hit
TGTCGATGAAGAACGCAGCTAGCTGCGAGAATT2487190.3728547740856315No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACG2408980.3611303091749342No Hit
TATGCTCGTGAGGGAAACAGCCCAGATCGTC2359460.353706763562126No Hit
AAGACGAGGCCGACAGGCGTAGTCGATGGAC2247030.33685237678409635No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2012000.30161901803251484No Hit
TAGGGTCGGGCCTGGTTAGTACTTGGATGGGAGAC1983830.2973960519599622No Hit
TGTACAGTGAAACTGCGAATGGCTCATTAAATC1942530.29120476694766456No Hit
ATTGCACTCCGGATGTGCTGACCCCTGCG1891860.2836088247788238No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATC1856790.27835147937007615No Hit
AAGACGAGGCCGACAGGCGTAGTCGATGGACAAC1856260.27827202704425247No Hit
AACTGCCTGTACCTAGAACGAACACACGTGGTC1805930.2707270542919779No Hit
AGCTATGTCGCGGTGAATACGTTCTCGGGTCTTG1743930.26143263127109523No Hit
ACCCCGAGAGGGGAGTGAAAGAGATCCTGAAACC1647340.2469528196648524No Hit
AGGGGAGCGTTCCAAGGGTGATGAAGTC1499770.2248305937746644No Hit
ATTGCACTCCGGATGTGCTGACCCCTGCGA1499600.22480510906638138No Hit
TAGGGTCGGGCCTGGTTAGTACTTGGATGGGA1460290.21891214505104428No Hit
TTTGTATAGTAGAAGCTATTGGGAAGTAGCGGCAT1428830.21419597491818998No Hit
CGTTGAAGTGTGATGGGGAGTGAACGGTTCGCCT1402550.21025633883772551No Hit
TAAAGATTAAGCCATGCATGTCTAAGTACGCA1363850.20445481995211004No Hit
TTGTATAGTAGAAGCTATTGGGAAGTAGCGGCAT1332740.19979111833630905No Hit
AAGGACTGGTGGAGCGCTTGGAAGTGATT1283810.19245601965224796No Hit
ACAAGTGGTGGAGCATGTGGTTTAATTCGA1227670.18404006951688742No Hit
TTGGCGCTGCGGGATGAACCGAACGCCGGGTT1204150.18051418517090098No Hit
TAAACTCAAAGGAATTGACGGGGACCCGCAC1203490.1804152445387432No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1185660.1777423483699958No Hit
TTGATGAAGAACGCAGCTAGCTGCGAGAATT1183720.17745152287547142No Hit
TGGACGGTGGCCATGGAAGTCGGAATCCGCT1180240.1769298359059122No Hit
TACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1138940.17073855089361456No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACGC1131490.1696217210306214No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTA1128960.16924244860734988No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1128600.1691884809898093No Hit
ATGTAGACCACATGGTTGATAGGATGGATGTGT1127640.16904456734303433No Hit
TAAAATGAGAGTTTGATCCTGGCTCAGGATAAAC1125450.16871626433632897No Hit
AAAACTCAAAGGAATTGACGGGGACCCGCAC1121870.16817958636189734No Hit
AGTCAGGACCTAAGACGAGGCCGACAGGCGT1113460.16691884285212924No Hit
TCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC1103000.16535078374247708No Hit
TGTATAGTAGAAGCTATTGGGAAGTAGCGGCAT1090380.16345891892758127No Hit
ATGTGAATTGTTGTGGAGGTAGAGCACTGAATCT1087040.16295821936484342No Hit
ATGTGAATTGTTGTGGAGGTAGAGCACTGAATCTA1079830.16187736791354398No Hit
ATGCTCGTGAGGGAAACAGCCCAGATCGTC1079110.16176943267846278No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTC1075930.16129271872352072No Hit
ATGAGTAACGTTTGGAAGTGAGAATCTTCC1064880.1596362126851215No Hit
AGGAAATGCCTCTATATTGACGGTACCTTACC1054170.15803067606328836No Hit
TAGGATTCCCTCAGTAACGGCGAGTGAAC1028770.15422296082570094No Hit
ATGAAGCCGGAATCACTAGTAATCGCGAATC994160.1490345740393663No Hit
AGCTAAGGTCCCTAAGTGTAAACTAAGTGTGT954610.1431056316123355No Hit
ACAGAAGTTAAGCTCTATTACGGTGAAAAT950640.14251048871890157No Hit
AGGTGAGGTAATGGCTCACCTAGGCGATGATACGT950170.1424400309960013No Hit
TACACGTGGTCAAGGAGAAAATCCTAAGGC939270.14080601146491065No Hit
AAGTGGTGGAGCATGTGGTTTAATTCGA930510.13949279943808918No Hit
AGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGC919790.1378857637157688No Hit
ACGTGCTACAATGGCTGGTACAAAGAGTCGC893720.13397760874553638No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC893480.13394163033384263No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT886490.13289375909326248No Hit
TAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG885680.1327723319537961No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC882250.1322581404866731No Hit
AACACGAAGAACCTTACCAGGGCTTGACATCC871970.13071706518579124No Hit
TATTCGTTGAAAAGACTTTGGATGACTTGTGGCT864170.12954776680574473No Hit
CCCCCAGTGGCGAACGGGTGAGTAACACGT851380.12763041728256586No Hit
CGGTGGTTCAAGTCCACTCAGGCCTACCA838090.12563811273502504No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG837640.1255706532130993No Hit
AAGACCACTTGGTAAGCCTAAATACTACC822110.1232425501564169No Hit
TAGACAGCTAGGATGTTGGCTTAGAAGCAGCCATC821600.1231660960315677No Hit
TGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGC815970.12232210245725207No Hit
AGATCGTCAGCTAAGGTCCCTAAGTGTAAACT811450.12164450903701997No Hit
TGATGGTAGTCGCCGTGCCTACCATGGTGACC809570.12136267814541901No Hit
TCGTTGAAGTGTGATGGGGAGTGAACGGTTCGCCT805710.12078402535734471No Hit
TAGACCACATGGTTGATAGGATGGATGTGT804890.1206610991173911No Hit
AGCGGAGGAGAAGAAACTAACCAGGATTCCCTC803830.12050219446574374No Hit
TAGAAAAGGTGTTGGTTGATATAGACAGC793980.1190255804858132No Hit
AACTAGACGGAAAGACCCCGTGGAGCTTTACT790240.11846491690358577No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC785990.11782779919650911No Hit
AAGGAGAAAATCCTAAGGCAAGCGAGATAACTGT785580.11776633607653232No Hit
TGTAATGATCCCTTCGCTGTCTCGGCTGC783610.1174710132805462No Hit
AAAAGAGTCGCAATCTCGCGAGGGGGAGCT780220.11696281821537213No Hit
AAAAATGAGAGTTTGATCCTGGCTCAGGATAAAC768660.1152298580521237No Hit
ATAGAGGTCACACCTGTTACCATACCGAACAC754180.11305916054660141No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGC743690.11148660413548757No Hit
AAAAGCTATAGAGATATAGTGGAGGTTAAC742950.11137567069943186No Hit
AGGAGCGGTAGTTCAGTCGGTTAGA742060.11124225075606758No Hit
TAAACTCAAAGGAATTGACGGGGACCCGC733130.10990355402096302No Hit
ACCTAGAGGAAAAGAAAATAATAATGATTCTGTT732680.10983609449903727No Hit
TGGAGAGGGAGCCTGAGAAACGGCTACCAC727290.10902807933641538No Hit
AGTAGGTGAGGTAATGGCTCACCTAGGCGATGAT726340.10888566479012765No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAA726070.10884518907697219No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT722510.10831150930351507No Hit
ATAGCTTATAAGAAGGGAACCTAGGGAACTGAAAC721250.10812262264212294No Hit
AGTACCGGTGAAGATGCCGGTTACCCGCAACT717000.1074855049350463No Hit
TTAAGATCCCATGTAGACCACATGGTTGAT715730.10729511917316693No Hit
ATATGACTCCACGCTTCCAAGAAAAGCTT714800.1071557028278537No Hit
AAGTCGCAAGACGATGTATAGGGGGTGACACC704940.1056775897474359No Hit
TAAAGATTAAGCCATGCATGTCTAAGTACGCACGG691350.10364031218527792No Hit
TTTGTATAGTAGAAGCTATTGGGAAGTAGCGGC689040.10329401997272566No Hit
ATGGGTGATAAGGTCCATGCTCGTGAGGGAAAC686110.10285478352996459No Hit
TTGTATAGTAGAAGCTATTGGGAAGTAGCGGC684300.10258344634177433No Hit
AGTGGCGAGCGAACACGGAACAGGCCAAACCG683470.10245902100133346No Hit
AAAACTCAAAGGAATTGACGGGGACCCGC677190.10151758589534729No Hit
ATGTACACGGAGTCGACCCAACGCGA667860.10011892514075317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)