FastQCFastQC Report
Fri 14 Jul 2023
SRR9077164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9077164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18164197
Total Bases926.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG10478245.768622747264853No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA6545553.603544929621717Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA6519973.589462281211771Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4269682.3506021213048944No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3935662.166712902309967No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG3917342.1566271275300526TruSeq Adapter, Index 2 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC3325621.8308654106757374No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC2451351.3495504370493228Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA2418431.3314268723247167Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2188461.2048206700246644No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1735080.9552197655640928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1576440.8678831219458807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC1513240.8330894010894069No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1332180.733409795104072No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG1230650.677514122974993Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1213060.6678302376923131No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1124250.6189373524191573No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1095740.6032416406846942No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1081870.595605740237237Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1063000.5852171720005019No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1048550.5772619620894884No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG977750.5382841861933121No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC879840.4843814455436703No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA876010.4822729020170834No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT850320.46812969491577305No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA790260.4350646494309658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC738850.4067617192216095No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC713870.39300939094637655No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT617980.34021872808360315No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG595840.3280299151126802No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC568830.31316000371500047No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA567890.3126425021706162No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC559890.3082382337077714No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG554510.30527636316650825No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA524700.28886495780683286Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGT510650.2811299613189617No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA467040.257121192860879No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC465310.25616876980578884No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT457670.2519626934237721No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT430040.23675145122022184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG404560.2227238561660612Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG403520.22215130126589133Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT384080.21144892890117853No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC375090.2064996322160567No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC351620.19357860961318576No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA348400.19180589155689073No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC343600.18916333047918388No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA340010.18718691500648224No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG312580.17208577951450318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA310600.1709957230699491Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA309740.1705222642101933No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC302760.1666795399763612No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA293860.16177979131144637Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT293530.161598115237354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT292920.16126228976706208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA287740.1584105259373701Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT275200.15150683512186086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT270900.1491395408230818No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT270490.148913822064361Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT269130.1481650964256774No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT267990.147537488169722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC262970.14477380970928688Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC258270.1421863019873656Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA255420.14061728134747714No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA240740.13253544871815692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA240390.13234276197290748Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACG238060.13106001878310392No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT233620.12861564978622506No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACAC221940.12218541783047168No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG215810.11881064712081683No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG214710.11820506020717568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC212940.11723061580977127No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACAC207490.11423020791945825No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT207300.11412560654346571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTC197690.1088349790524734No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC195560.10766234257424097No Hit
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC195260.10749718250688428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGAGATCGGAAGAGCACAC194930.10731550643279195No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG193770.10667688750567944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG186140.10247631645924121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)