FastQCFastQC Report
Fri 14 Jul 2023
SRR9077166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9077166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32740120
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA18181075.553147025728678Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG16707835.1031670012205215No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA14253344.35347824015306Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC10996193.358628496169226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC10707933.270583614232324No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG6105521.8648435008790438No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC5559691.698127557260022No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG5239841.6004339629787552No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4986341.523006024412861No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA4386991.3399431645333004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA3895911.1899498230305814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA3805581.1623598203060954Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG3215160.9820245008265088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA3201200.9777606190814205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA2968680.9067407205593627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC2251140.687578420604445Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG2167460.6620195649863226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2021150.6173312742897704No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA2013790.6150832678682913No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1885230.5758164600496272No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1867070.5702697485531513No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC1791490.5471849217412765No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1602700.48952172441640407No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1586530.4845828298735619No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1572940.48043195932085775No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTC1520220.46432939158439246No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCT1471500.44944856646829634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1436800.4388499492365941No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT1377390.4207040169675615Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA1293690.3951390526363373Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1274230.38919527478824145No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1264500.3862233858641935No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG1114480.3404019288872491Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1099540.3358387202001703No Hit
GGCGGGCGTGGGGGTGGGGGCCGGGCCGCAGATCGGAAGAGCACACGTCT1062300.32446429640453367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1049570.32057610051520885No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT996440.3043483041601558No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAA979080.29904594118775374Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA955950.2919812144854692No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT938630.2866910689392709No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC933230.2850417164017725No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG894170.2731113997138679TruSeq Adapter, Index 4 (100% over 49bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA863930.2638750255038772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACAC848850.2592690558250856No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACG819140.25019456251229377No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGT770820.2354359116582346No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA755590.23078412663117914No Hit
GCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT710710.21707617443063743Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC699010.21350257726605765No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC691750.21128511441008768No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT652360.1992540039560026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG652160.19919291682498416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC620670.18957474804612812No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACA610850.18657536991312187No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG594210.18149292061238625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA593750.1813524202110438No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC573980.17531395730986937No Hit
CCCCGCCCCTTGCCTCTCGGCGCCAGATCGGAAGAGCACACGTCTGAACT558500.1705858133690408Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT557730.17035062791461972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC542130.1655858316951801No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC532370.1626047797014794No Hit
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA517610.15809654943231727Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC515550.1574673519828272Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTC512020.15638916412035142No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTGAA506450.15468788752148738Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG498100.15213749980146685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC493870.15084550698042648No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC490570.14983756931862194No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAAC487170.14879908809130815Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT465530.1421894605151111Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACG465510.14218335180200928No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT463910.14169465475386164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT459760.14042709678522863No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG445600.13610212790912188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT430890.1316091694227144No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC408730.12484071530586938No Hit
GGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTC398050.12157866250948378Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAGATCGGAAGAGCAC394960.12063486633524861No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG392840.11998734274645297No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACAC391920.11970634194376807No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG385180.11764770562844608No Hit
CGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAAC379720.11598002695164221Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCTGA377610.11533555771939749Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA375740.11476439304437493No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA374500.1143856528320605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT358850.10960558482986622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGA348540.10645654322586479Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG348480.10643821708655925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCAGATCGGAAGAGCACACGTCTGA341320.1042512977960985Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC337210.10299595725366918No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT328150.1002287102185331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)