FastQCFastQC Report
Fri 14 Jul 2023
SRR9077186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9077186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26431823
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA15556115.885371584093916Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG15512775.868974682525682No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA12144594.5946849750015355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC9039643.419983555428621No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC7593932.8730254436101514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC5203401.9686118509495163No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4824881.8254056861685248No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3913181.4804805555787808No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3843691.4541902766222368No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA3699601.3996764430512418Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG2768921.0475705743035584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA2439140.9228043029797831Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA2294380.8680369870818219Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1994910.7547379535645347Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG1802330.6818788094941466Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC1748580.6615434735621527No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1643730.6218753810510913No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1397190.5286014513641378No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1376390.5207321492732454No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1343850.5084212314829741No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1262350.47758718723260213No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1192670.45122502522811236No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC1181850.44713147481352306Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1169930.4426217593845116No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA1142320.43217601752251444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1134300.42914179623554527No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1085800.4107927024178393No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1001970.3790771450005548No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA988010.3737956326357058No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT913970.3457839438467789Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA885180.33489177042385615No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT882160.3337492082933516No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA833250.3152449984248154Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA806110.3049770725235259Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGCGTGGGGGTGGGGGCCGGGCCGCAGATCGGAAGAGCACACGTCT790360.2990183461806626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC737190.2789024427108187No Hit
GCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT732380.2770826666022998Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG729100.2758417381956591Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACAC725920.27463864297214763No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC716710.27115420680594No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA713090.2697846455766596No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACG675450.25554423544679455No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT664610.2514431183955794No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG654030.24744036762050048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC649200.24561302487535572Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC610410.23093753313950383No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT609570.2306197344012178Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACA600430.22716178146320065No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGT592580.2241918765875513No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC584440.22111225548082702No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT564900.21371965149736363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT558380.21125292795733386Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA519100.19639205362414844No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC512940.19406152954338413No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG512330.19383074712629544Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC488880.18495886568247677No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAA482520.18255267523545388Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC477520.18066101607898932No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT471540.17839859172785774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGCGTGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTC468280.17716522995784287No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG465260.17602266782733827No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC443910.1679452832292347No Hit
CCCCGCCCCTTGCCTCTCGGCGCCAGATCGGAAGAGCACACGTCTGAACT442090.1672567192962816Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC414850.1569509602118628No Hit
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA413880.1565839783355087Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACG405690.15348544063721975No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT399390.15110195010007446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAAC388070.14681923376983874Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACAC384420.14543832258561962No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG383180.1449691911148164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA380470.14394391185201263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC370090.14001682744319224No Hit
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC338240.12796695861651314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGC337360.1276340266049754No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTC332820.12591640009090557No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA324450.12274976266298393No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA308250.11662078699603882No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGAGATCGGAAGAG299570.1133368667004164No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAAC297010.11236833721230656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT288780.10925466624076591No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT285260.10792293819461489No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAGC277180.10486601699776818No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACAC276750.10470333431031223No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCT274360.1037991212335222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT273630.10352293899667836No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC268810.10169937956984654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)