FastQCFastQC Report
Fri 14 Jul 2023
SRR9080408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9080408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82589835
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-37
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAAT78862699.548716255456862No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT19056762.307397756636758No Hit
GTGCCCGAGTTGTAATTTG18936762.292868123540869No Hit
TGTGCCCGAGTTGTAATTTG18911702.289833851829344No Hit
TCACGTTCTAGCATTCAAGGTC16665872.0179081360315108No Hit
TATAGATGGATACGAATAAGGCGTC13165281.5940557333720307No Hit
TAGATGGATACGAATAAGGCGTC12970841.5705128845456586No Hit
TCACGTTCTAGCATTCAAGGTCC12298921.489156625630745No Hit
TTATAGATGGATACGAATAAGGCGTC9359951.1333053274655411No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT8948971.0835437557176375No Hit
AGATGGATACGAATAAGGCGTC8755271.060090506295357No Hit
TCACGTTCTAGCATTCAAGGTCCC8626911.044548642093788No Hit
TTAGATGGATACGAATAAGGCGTC7572760.9169118693601942No Hit
AAATATAGATGGATACGAATAAGGCGTC6282000.7606262925697818No Hit
ATATAGATGGATACGAATAAGGCGTC5831650.7060977903636688No Hit
TATATAGATGGATACGAATAAGGCGTC5805750.7029618112204729No Hit
CACGTTCTAGCATTCAAGGTC5293870.6409832396444429No Hit
ACGTTCTAGCATTCAAGGTC4830580.5848879586694901No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA4309860.5218390374553963No Hit
CACGTTCTAGCATTCAAGGTCC3802790.4604428620059599No Hit
ATAAATCATTTGTATACGACTTAGAT3461450.41911332066470414No Hit
ATAGATGGATACGAATAAGGCGTC3408350.4126839580197733No Hit
AAGAGGTGCACAATCGACCGATCCTGA3255350.39415867582251496No Hit
ATATTAGCATGGAATAATAGAAT3109390.37648579876688215No Hit
TTGTGGCGTCGCTGAACCATA3079670.3728872929701337No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG3066540.37129750894889185No Hit
ACGTTCTAGCATTCAAGGTCC2911250.3524949529200537No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA2862420.3465826030527849No Hit
ATTAGCATGGAATAATAGAAT2725710.33002971854829355No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATA2662570.32238470993434No Hit
TTTCTTTACTTATTCAATGAAGCGGAG2502960.3030590871140498No Hit
CACGTTCTAGCATTCAAGGTCCC2495370.30214008782073487No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2340810.28342592039323No Hit
AAGAGGTGCACAATCGACCGATCCTG2329630.2820722429097964No Hit
GCGCAGTGGAAGCGCCAGGG2235070.27062289203023593No Hit
TATTAGCATGGAATAATAGAAT2217020.26843739305206266No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT2208690.26742879435465633No Hit
TCTTCTTCATGATAAGACAGTT1922890.23282405153128108No Hit
TTTGCTCGAATATATTAGC1801660.21814548969615932No Hit
ACGTTCTAGCATTCAAGGTCCC1719620.20821206386960334No Hit
AATAGATGGATACGAATAAGGCGTC1701500.20601808927212412No Hit
TTGCTCGAATATATTAGC1638410.19837913467196053No Hit
TCAATATAGATGGATACGAATAAGGCGTC1574810.19067842913113944No Hit
TCAGATTTAGGCTCTGATA1565580.189560858185514No Hit
CCTGATTCAAGCTCAGGT1488440.18022072571037345No Hit
CCACGTTCTAGCATTCAAGGTC1470820.17808729125079376No Hit
AGCATGGAATAATAGAAT1464570.17733053952704952No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGA1447260.17523463995296756No Hit
TTTGCTCGAATATATTAGCATG1384940.16768891716516932No Hit
TAGCATGGAATAATAGAAT1382440.16738621647567162No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG1373110.16625653750246625No Hit
ATATATAGATGGATACGAATAAGGCGTC1351880.1636860032472519No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1351740.16366905200864004No Hit
TAAGGCGGCAGACTTAAGATCTGTTG1285020.15559057600732584No Hit
GGTGCCCGAGTTGTAATTTG1247980.15110576259172814No Hit
CCACGTTCTAGCATTCAAGGTCC1244620.15069893286504327No Hit
ACAATCGACCGATCCTGA1239930.15013106637154558No Hit
CGTGCCCGAGTTGTAATTTG1229390.14885488026462337No Hit
TCAAACTTTAAATATGTAAGAAGT1229190.14883066420946356No Hit
TTTGCTCGAATATATTAGCATGGAATA1226140.14846136936827636No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA1221740.14792861615476044No Hit
TTAGCGACATTGCTTTTTGATT1188060.14385063246584764No Hit
TAAGGAGGCAGACTTAAGATCTGTTG1184460.14341474347297098No Hit
GCGCAGTGGAAGCGCTCAGGG1141500.13821313482464276No Hit
TTGTGGCGTCGCTGAACCATAGC1113110.13477566579470707No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA1109400.1343264579714925No Hit
ATGCCTTGCTAAGGCATTG1095060.13259016681653377No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAT1077120.13041798666869842No Hit
ATGGATACGAATAAGGCGTC1074730.1301286048095386No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA1057980.12810051018990412No Hit
GATGGATACGAATAAGGCGTC1052690.12745999553092702No Hit
CCACGTTCTAGCATTCAAGGTCCC1017480.12319675902004164No Hit
GCGCAGTGGAAGCGCGCAGGG1000220.12110691345974961No Hit
TTGTGGCGTCGCTGAACCATAG1000220.12110691345974961No Hit
TCTTTACTTATTCAATGAAGCGGAG993460.12028841079534788No Hit
TAGATGGATACGAATAAGGCGT977830.11839592608460835No Hit
TAGATGGATACGAATAAGGCGTCC973060.11781837316904677No Hit
TTTAGGGATAACAGGGTAAT972400.11773846018701938No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT969030.1173304196575765No Hit
TTTAGCATGGAATAATAGAAT918090.11116259040837169No Hit
TTTGTGGCGTCGCTGAACCATA902990.10933427824380566No Hit
GCTCGAATATATTAGCATGGAATAATAGAAT888870.10762462474952275No Hit
AGTGCCCGAGTTGTAATTTG886840.10737883178965062No Hit
AGATGGATACGAATAAGGCGTCC883930.10702648818707533No Hit
GTTTAGTGGTAAAATCCAACG869980.10533741833967825No Hit
TATAGATGGATACGAATAAGGCGT861740.10433971686709387No Hit
TATAGATGGATACGAATAAGGCGTCC861460.10430581438987012No Hit
TGTGGCGTCGCTGAACCATA856260.10367619695571496No Hit
TGATGGATACGAATAAGGCGTC847800.10265185782245478No Hit
CTCGAATATATTAGCATGGAATAATAGAAT846210.10245934018393425No Hit
TAATATAGATGGATACGAATAAGGCGTC842250.10197986229176993No Hit
AAGAGGTGCACAATCGACCGATCCT831060.10062497400557828No Hit
AGCTCAAATTTGAAATCTGG826300.10004863189277469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)