FastQCFastQC Report
Fri 14 Jul 2023
SRR9080409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9080409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81516240
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length18-37
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1275319715.644977000901905No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT28104783.4477522515758823No Hit
TCACGTTCTAGCATTCAAGGTC16562032.031746066796015No Hit
TAGATGGATACGAATAAGGCGTC10693321.3118024089432978No Hit
TCACGTTCTAGCATTCAAGGTCC10363021.2712828756576604No Hit
TATAGATGGATACGAATAAGGCGTC9808421.2032473529201053No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA9476431.1625204989827795No Hit
GTGCCCGAGTTGTAATTTG8594621.054344508529834No Hit
TCACGTTCTAGCATTCAAGGTCCC8019930.9838444462109636No Hit
TTATAGATGGATACGAATAAGGCGTC7419130.9101413411609761No Hit
TGTGCCCGAGTTGTAATTTG7413090.9094003845123377No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT6932600.8504563017136217No Hit
TTAGATGGATACGAATAAGGCGTC6679320.8193851924475417No Hit
AAGAGGTGCACAATCGACCGATCCTGA6136160.7527530710444937No Hit
AAGAGGTGCACAATCGACCGATCCTG5584350.6850598113946375No Hit
CACGTTCTAGCATTCAAGGTC5565730.6827756039778086No Hit
AGATGGATACGAATAAGGCGTC5196280.637453346719623No Hit
ACGTTCTAGCATTCAAGGTC4984980.6115321315114632No Hit
TATTAGCATGGAATAATAGAAT4892340.6001675249005597No Hit
AAATATAGATGGATACGAATAAGGCGTC4660800.5717633688698105No Hit
TATATAGATGGATACGAATAAGGCGTC4356070.5343806338467034No Hit
ATATAGATGGATACGAATAAGGCGTC4308900.5285940568406983No Hit
TTTAGCATGGAATAATAGAAT3524700.43239236745953935No Hit
CACGTTCTAGCATTCAAGGTCC3480150.4269271988011223No Hit
ATTAGCATGGAATAATAGAAT2992370.3670888156764836No Hit
TTGTGGCGTCGCTGAACCATA2917410.357893102037091No Hit
ACGTTCTAGCATTCAAGGTCC2905340.3564124154892326No Hit
ATATTAGCATGGAATAATAGAAT2851200.34977079413868944No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATA2794440.3428077644405581No Hit
TAGCATGGAATAATAGAAT2737460.33581774625522476No Hit
CACGTTCTAGCATTCAAGGTCCC2608870.32004297548562105No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA2556590.3136295295268771No Hit
ATAGATGGATACGAATAAGGCGTC2457940.30152764651559No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG2398530.2942395282216157No Hit
ATAAATCATTTGTATACGACTTAGAT2300570.28222229092018963No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGA2289710.28089004105194254No Hit
TTGTGGCGTCGCTGAACCATAG2286820.280535510470061No Hit
ACGTTCTAGCATTCAAGGTCCC2053690.25193630128180594No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA2045560.250938954004748No Hit
TTTCTTTACTTATTCAATGAAGCGGAG1952800.23955962640082518No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT1872910.2297591252982228No Hit
TTGTGGCGTCGCTGAACCATAGC1802080.22107005916857791No Hit
TTAGCGACATTGCTTTTTGATT1662430.20393850354236162No Hit
AGCATGGAATAATAGAAT1652790.2027559171031441No Hit
TTAGCATGGAATAATAGAAT1612830.1978538264277155No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1569260.19250887921228949No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAT1559050.19125636805623025No Hit
CCACGTTCTAGCATTCAAGGTC1464690.17968076054538334No Hit
ACAATCGACCGATCCTGA1358680.16667598996224556No Hit
TCTTCTTCATGATAAGACAGTT1356590.166419599333826No Hit
AAGAGGTGCACAATCGACCGATCCT1323360.16234311101689675No Hit
GCTCGAATATATTAGCATGGAATAATAGAAT1286210.15778573692800357No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1266210.1553322380914527No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1264090.15507216721477832No Hit
AATAGATGGATACGAATAAGGCGTC1226640.15047799064333683No Hit
AAAGAGGTGCACAATCGACCGATCCTG1225270.1503099259730331No Hit
TAAGAGGTGCACAATCGACCGATCCTG1218250.14944874788140375No Hit
TTTGCTCGAATATATTAGCATG1172690.14385967753174092No Hit
CTCGAATATATTAGCATGGAATAATAGAAT1169410.1434573037225466No Hit
GCGCAGTGGAAGCGCCAGGG1165680.14299972618952983No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG1114000.13665988519588243No Hit
TCAATATAGATGGATACGAATAAGGCGTC1113500.13659854772496868No Hit
TGTGGCGTCGCTGAACCATAG1088520.13353412767811668No Hit
TTTGCTCGAATATATTAGC1082430.13278703728238694No Hit
CCTGATTCAAGCTCAGGT1055700.12950793608733668No Hit
CCACGTTCTAGCATTCAAGGTCC1049660.12876697943869836No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA1028470.12616749742137273No Hit
ATATATAGATGGATACGAATAAGGCGTC1024320.12565839641278842No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA996170.1222050968003431No Hit
TAGATGGATACGAATAAGGCGT986360.1210016556210149No Hit
TCAGATTTAGGCTCTGATA954560.11710059247089905No Hit
GCGCAGTGGAAGCGCCAGGGC939410.11524206710221178No Hit
TCAAACTTTAAATATGTAAGAAGT925210.11350008292826066No Hit
TTTGCTCGAATATATTAGCATGGAATAATAG918810.11271496330056441No Hit
TTTGTGGCGTCGCTGAACCATA915250.11227824050765835No Hit
TTGCTCGAATATATTAGC913830.11210404209026324No Hit
CCACGTTCTAGCATTCAAGGTCCC891190.10932668140728767No Hit
TTTGCTCGAATATATTAG886880.10879795240801096No Hit
TGTGGCGTCGCTGAACCATA883310.10836000286568664No Hit
ATGCCTTGCTAAGGCATTG857840.10523547209733913No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA849190.10417433385053088No Hit
TATAGATGGATACGAATAAGGCGT830820.10192079516915893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)