FastQCFastQC Report
Fri 14 Jul 2023
SRR9080410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9080410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87883890
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-37
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAAT64219167.3072732670344935No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT14775791.6812853868894515No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT10380791.1811937318659882No Hit
TTTAGGGATAACAGGGTAAT5841310.6646622037326749No Hit
TCAGGTCGAGGTCCTGATC5373290.6114078473312914No Hit
TCACGTTCTAGCATTCAAGGTCCC5218120.5937515965667883No Hit
TCACGTTCTAGCATTCAAGGTC5178740.5892706843085803No Hit
TTTGCTCGAATATATTAGCATG5010020.5700726265075431No Hit
TCACGTTCTAGCATTCAAGGTCC4755450.541105997925217No Hit
AAGAGGTGCACAATCGACCGATCCTGA4583180.5215039980592575No Hit
ATGTATTTATTAGATAAAAAATCA4258390.48454728164627214No Hit
ATATTAGCATGGAATAATAGAAT4098350.4663368906405941No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA4083090.4646005086939142No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATA3866730.4399816621681175No Hit
TGTGCCCGAGTTGTAATTTG3681580.41891409221872183No Hit
TCAGATTTAGGCTCTGATA3597740.4093742322967269No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT3148900.35830230091089504No Hit
TTGTGGCGTCGCTGAACCATAG3033110.34512696240459995No Hit
CCTGATTCAAGCTCAGGTA2736520.3113790252115604No Hit
ATTAGCATGGAATAATAGAAT2610930.2970885790330856No Hit
TTTGTGGCGTCGCTGAACCATAG2558590.2911329937716685No Hit
GTGCCCGAGTTGTAATTTG2542790.28933516711652163No Hit
TCACGTTCTAGCATTCAAGGTCCCA2063330.2347790931876138No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA2006330.22829326284942555No Hit
TATTAGCATGGAATAATAGAAT1952210.2221351376230615No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC1851870.21071780049790695No Hit
ATGTATTTATTAGATAAAAAATCAATG1817170.20676940904641342No Hit
AAGAGGTGCACAATCGACCGATCCTG1816090.20664651962947927No Hit
ATAAATCATTTGTATACGACTTAGAT1767170.20108008418835352No Hit
TTTGCTCGAATATATTAGCATGGAAT1679580.19111352490200423No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAAT1615120.18377884729499344No Hit
ATATTAGCATGGAATAATAGAATA1556210.17707568474722726No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1488180.169334789345351No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1303710.1483445942140249No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGA1232510.14024299561614764No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1182130.1345104318891665No Hit
TTTGCTCGAATATATTAGCATGGAATA1140650.12979056798692No Hit
TTGTGGCGTCGCTGAACCATA1067820.12150349739866999No Hit
TATAGATGGATACGAATAAGGCGTC1052530.11976370185707529No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1036790.11797270239175804No Hit
ATGCCTTGCTAAGGCATTG1033330.11757900111158029No Hit
ACGTTCTAGCATTCAAGGTC1021590.11624314763490783No Hit
ATCCTGTCTGTGACGCCA1012510.11520996624068416No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1004980.11435315391706034No Hit
TTATAGATGGATACGAATAAGGCGTC989520.11259401467094822No Hit
TTTGCAAGGATTGATAATACAACT989380.11257808456134565No Hit
TAGATGGATACGAATAAGGCGTC962380.10950584913799331No Hit
TCGGTGCCCGAGTTGTAATTTG961940.10945578307924239No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT951320.10824737047939047No Hit
TTGTGGCGTCGCTGAACCATAGC944490.1074702087037795No Hit
TTTGCTCGAATATATTAGCA944150.10743152129474469No Hit
CCACGTTCTAGCATTCAAGGTCCC935850.10648709336830676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)