FastQCFastQC Report
Fri 14 Jul 2023
SRR9080411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9080411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98675833
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-37
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAAT73177587.415957664122278No Hit
TTTAGGGATAACAGGGTAAT35999233.648231679989973No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT12174481.2337853788373896No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT11167561.1317421561569185No Hit
TCACGTTCTAGCATTCAAGGTCCC9883081.00157046558705No Hit
TCACGTTCTAGCATTCAAGGTCC6645300.6734475704907401No Hit
TCACGTTCTAGCATTCAAGGTC6348420.643361176388549No Hit
AAGAGGTGCACAATCGACCGATCCTGA5087210.515547712680571No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATA4845970.49109998392412857No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA4661270.4723821282562672No Hit
ATGTTAGGGATAACAGGGTAATGCGA4518430.45790644604945974No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA4430140.44895896647763794No Hit
TCAGGTCGAGGTCCTGATC4362680.4421224394426952No Hit
ATGTATTTATTAGATAAAAAATCA3886900.39390597290422674No Hit
ATGGAGAACAGAAATCTCCAGTAGAAC3860590.3912396665554371No Hit
TTTGCTCGAATATATTAGCATG3731580.37816554333014857No Hit
TCACGTTCTAGCATTCAAGGTCCCA3624630.3673270232236094No Hit
TTGTGGCGTCGCTGAACCATAG3025520.3066120556590589No Hit
TCAGATTTAGGCTCTGATA2910800.2949861087060699No Hit
TGTGCCCGAGTTGTAATTTG2564700.25991166449033165No Hit
TTTGCTCGAATATATTAGC2527200.2561113418723306No Hit
TTTGTGGCGTCGCTGAACCATAG2443310.2476097668210209No Hit
ATATTAGCATGGAATAATAGAAT2425380.24579270589993396No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT2329050.23603043715881272No Hit
ATTAGCATGGAATAATAGAAT2262360.22927194341495954No Hit
CCTGATTCAAGCTCAGGTA2086870.21148744698207916No Hit
AAGAGGTGCACAATCGACCGATCCTG1912980.19386509764756688No Hit
ATGTATTTATTAGATAAAAAATCAATG1842000.18667184699621436No Hit
CCACGTTCTAGCATTCAAGGTCCC1770680.17944414008645868No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1729140.17523439604507823No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAAT1718700.17417638622822673No Hit
TTAGGGATAACAGGGTAAT1694670.17174113949461162No Hit
TTTGCTCGAATATATTAGCATGGAAT1675770.16982577689513906No Hit
GTGCCCGAGTTGTAATTTG1616550.1638243074167917No Hit
ATAAATCATTTGTATACGACTTAGAT1574910.1596044291817633No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1469980.14897061978691378No Hit
AGTTATCGTTTATTTGATAGTT1411400.14303400914791364No Hit
TATTAGCATGGAATAATAGAAT1410600.14295293559872962No Hit
TATAGATGGATACGAATAAGGCGTC1401310.14201146900883016No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC1383730.14022987776551124No Hit
TTATAGATGGATACGAATAAGGCGTC1321530.1339264093164534No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1319160.13368622892699572No Hit
TTTGCTCGAATATATTAG1305260.13227757600992332No Hit
AAATATAGATGGATACGAATAAGGCGTC1302540.13200192594269763No Hit
TTTGCTCGAATATATTAGCATGGAATAAT1269200.12862318578045345No Hit
ACGTTCTAGCATTCAAGGTCCC1225030.12414691244613055No Hit
CACGTTCTAGCATTCAAGGTCCC1213600.12298857411216382No Hit
TTTTCCACGTTCTAGCATTCAAGGTC1205460.12216365074921638No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1201340.12174612197091866No Hit
TCCACGTTCTAGCATTCAAGGTCCC1199360.1215454649366882No Hit
ACGTTCTAGCATTCAAGGTCC1168440.11841197226072568No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1162280.1177877059320087No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1155870.11713810411917171No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1152370.1167834073414916No Hit
ACGTTCTAGCATTCAAGGTC1150740.11661821998502916No Hit
TTGTGGCGTCGCTGAACCATAGC1134190.11494101093628467No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1128060.1143197848656621No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1105770.11206087310152223No Hit
TCGGTGCCCGAGTTGTAATTTG1088040.1102640805677313No Hit
ATATTAGCATGGAATAATAGAATA1086820.11014044340522566No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGA1078680.10931552004227822No Hit
TTTGCAAGGATTGATAATACAACT1072420.10868111951991324No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC1062570.10768290144558496No Hit
ATGTTAGGGATAACAGGGT1025810.10395757186057908No Hit
TAAGGCGGCAGACTTAAGATCTGTTG1021180.10348835869467654No Hit
CCACGTTCTAGCATTCAAGGTCC1010390.10239487920005702No Hit
TTTTCCACGTTCTAGCATTC990460.10037513440601002No Hit
ATCCTGTCTGTGACGCCA989170.1002444033079508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)