FastQCFastQC Report
Mon 24 Jul 2023
SRR9113067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9113067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15031439
Total Bases395.1 Mbp
Sequences flagged as poor quality0
Sequence length14-40
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGATGTACACTGACTG3714222.4709676831339964No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2993171.9912730910194294No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG2436801.621135541314441No Hit
TCGCTTCTGGCGCCAAGCGCCCGG1785581.1878969139282007No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1747751.1627296628087305No Hit
AGTCACTTAGCGATGTACACTGACTGTG1439340.9575530326803707No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1161980.7730331074756049No Hit
CCCGCGGACGCTACGCCGCGACGAGT1153600.7674581255992856No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG866270.5763054355607603No Hit
AACGATGCCGACCGGCGATGCGGCGGCG854730.5686281932155663No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG797890.5308141156678347No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG775400.51585214163461No Hit
CCGCGGACGCTACGCCGCGACGAGT745940.4962532196684562No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC705550.46938287146027735No Hit
TAGTCACTTAGCGATGTACACTGACTGTG697160.4638012368609552No Hit
AGCGATGTACACTGACTGTG696850.4635950024478694No Hit
ACTGACCCGGTGAGGCGGGG695810.46290311925558153No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG677390.4506488034844834No Hit
CCCCCGCGGGGGCGCGCCGGC627330.4173452721326282No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG618010.41114493429404864No Hit
GAGGGCGCACCACCGGCCCGTCTCGC610600.40621526654899776No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT568990.37853328613448123No Hit
CGCTTCTGGCGCCAAGCGCCCGG559080.3719404376387384No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG541140.3600054525717731No Hit
AGCTAGGAATAATGGAAT526080.34998645172960485No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC522950.3479041494297386No Hit
GCTTCTGGCGCCAAGCGCCCGG504760.335802846287704No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG456630.30378329047538294No Hit
CGCTATTAAAGGTTCGTTTGT414960.2760613937228498No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA411890.27401900776100013No Hit
TCGCTTCTGGCGCCAAGCGCCCG369540.2458447258442788No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC364500.24249175345088386No Hit
TTAGCAGTAAACTGAGAG350350.2330781504019675No Hit
CCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG341050.22689111800939352No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT336920.22414354340925044No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC334970.22284626242371075No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG332550.22123630345704093No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGC328190.2183357162278342No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT325280.2163997738340288No Hit
ACGGAGTCGAGCACCCGCAACGCGACTG324750.21604717951488212No Hit
CTCCCGGGGCTACGCCTGT314650.20932792928208668No Hit
GCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG309220.20571550069158384No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC305230.2030610642134795No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT297630.19800499473137603No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG293840.19548361271332707No Hit
GAGGGCGCACCACCGGCCCGTCTCG287950.19156515886469686No Hit
ACACGGAGTCGAGCACCCGCAACGCGACTG270210.1797632282577869No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG265620.17670962839951648No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG264420.17591130163918436No Hit
CCCCGCGGGGGCGCGCCGGC264100.1756984145030958No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC259920.17291757628793888No Hit
TCGACTGCATAATTTGTGGTAGTGGGG252390.1679080758668548No Hit
ATTAAAGGTTCGTTTGT250130.16640456046822927No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG232110.15441635361724185No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC231390.15393735756104254No Hit
ACCATGATCACGAAGGTGGT229060.15238727310139766No Hit
CTCCCGGGGCTACGCCT220910.146965303854142No Hit
TTCTGGCGCCAAGCGCCCGG219210.14583434094367145No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG218190.14515576319738915No Hit
CCCCGCGGACGCTACGCCGCGACGAGT208370.13862278920867124No Hit
CCCCCCGCGGGGGCGCGCCGGC205130.1364673069557745No Hit
TTAGCAGTAAACTGAGAGT188130.12515767785106935No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTT183870.12232361785189029No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC182610.12148537475354157No Hit
TCCCCGCGGGGGCGCGCCGGC181690.12087332423728692No Hit
AACCCGGCGGCGGGTCCGGCCGTG177900.11835194221923796No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT174480.1160767109522914No Hit
CGGCCGAGGTGGGATCCCG174400.11602348916826925No Hit
TGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG172070.11447340470862437No Hit
AGCTAGGAATAATGGAA171150.11386135419236974No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT169120.11251085142280788No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG169030.11245097691578297No Hit
AGTCACTTAGCGATGTACACTGACTGT167210.11124018132927925No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC166770.11094746151715748No Hit
ACTTAGCGATGTACACTGACTGTG160690.1069026059314747No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT159510.10611758461714808No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTA156610.10418829494634545No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCG155000.10311720654289985No Hit
CGCTTCTGGCGCCAAGCGCCCG154000.10245193424262308No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC151500.10078875349193114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)