FastQCFastQC Report
Fri 14 Jul 2023
SRR9132358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24120855
Total Bases964.8 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG9656534.003394572870655No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA9124263.782726607327974No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA8282213.4336303584595154No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG7484963.103107248893126No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA5983942.4808158748933233No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4369461.8114863672950232No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3263311.3528998039248608No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC3190521.322722598349022No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2373300.9839203461071342No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2212580.9172892088609629No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1659100.6878280226799589No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1559140.6463867056122181No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1544620.640367018499137No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1261520.5229997029541449No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1203990.4991489729530732No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1154770.4787433944609344No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA1095960.45436200333694643No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1066660.44221483857019167No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1013520.4201841103891218No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA961980.3988167086116972No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG956700.39662773147966773No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC869240.3603686519404059No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG865460.35880154331179387No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA855610.35471794013935243No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA848720.3518614908136548No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA832660.34520335203706504No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC827070.34288585541432925Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA802800.3328240230290344No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG790750.3278283460515807No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA786900.3262322168928091Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC779260.3230648333154028No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG771110.3196860144468345No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG728800.3021451768604388No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA714010.29601355341674246No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA676500.28046269504128274No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA620880.25740381093456266No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA590020.24460990292425375No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC578100.23966812121709616No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA566470.2348465674205993No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG547620.227031753227653No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA545040.22596213940177493No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC529020.21932058378527625Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA520380.21573862120559162No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG490260.20325150165696862No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA483480.2004406560215216No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA480130.19905181636388924No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA476770.19765883091623412No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGG475980.19733131350443425No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA474530.19673017395113065No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG462810.19187130804442878No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA438690.18187166250947573No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG433800.17984437118833474Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA431590.17892815159329967No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGA426570.1768469650018625No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA421620.1747947989405848No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG412590.1710511505500116No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA409850.169915204083769No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA389630.1615324166577014No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG384740.15950512533656042No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG382750.15868011312202657No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG378770.15703008869295884No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA378580.15695131868252596No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG372130.15427728411783082No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA360580.14948889664151624No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG356990.14800055802333706No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG349210.14477513338561174No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA344490.1428183204948581No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA340680.1412387744961777No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG334630.13873057153239385No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA324510.13453503202933728No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA317680.13170345744377635No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG309030.1281173490740689No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG297530.12334969054786822No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG295200.12238372147255976No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA290790.12055542807251235No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA284790.11806795405884245No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG279850.11601993378758754No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG273550.11340808607323413No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA270290.11205655852580682No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG268580.1113476284319109No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA268460.1112978789516375No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAG267790.11102011102011103No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATC265130.10991733087405069No Hit
GGGGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAA264820.10978881138334441No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG255870.10607832931295345No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGAGATCGGAA255740.10602443404265727No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG255320.10585031086170038No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG255190.10579641559140421No Hit
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTA254570.10553937660999166TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA253030.10490092494648304No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA246480.10218543248156003No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA245210.10165891714866658No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)