FastQCFastQC Report
Fri 14 Jul 2023
SRR9132359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24048949
Total Bases961.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG9282433.859806929608441No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA8846523.6785474492045367No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA8182393.402389850799717No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG7613633.1658888710687525No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA5893742.45072664090227No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4327091.7992844510585475No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3250431.3515892108216454No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2970001.2349812043761246No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2193470.9120855967551846No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2179700.9063597748076225No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1676780.6972362908665988No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1462460.6081180512295985No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1435260.5968077856541673No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1216350.5057809387013129No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1173850.48810864873970167No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1116280.46416997266699683No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA1114190.46330091181947286No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1028020.4274698241490719No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA971900.4041340850279985No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG923850.3841540019066946No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA914670.38033678727498654No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA848460.3528054386077329No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC845110.35141244634017066No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG836990.3480359994110345No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA817060.3397487349655072No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC792310.3294572249290395Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA777640.3233571662528787Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA763060.3172945312495777No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA758400.3153568166326104No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG753280.31322782546547046No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG747430.3107952867295781No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC734290.3053314304920352No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG707500.29419165053741014No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA675780.2810018849472382No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA642200.2670386967846287No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA605230.2516658836109636No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA585240.24335367005019637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG545290.22674171748628186No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC535920.22284549732298076No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA526040.21873720968014027No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC515230.21424221075108107Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA510000.21206747953933452No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG503150.20921912221611014No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA494220.20550586223123513No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA490160.2038176387666671No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA489280.2034517184098149No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA485580.2019131896366864No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA477830.19869059558486318No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGG465040.19337227585288655No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG455100.1892390390948062No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA433140.18010766291699484No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG420020.17465212305119862Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA406630.16908431216682276No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA395860.1646059459812568No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGA391290.16270565503714945No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG388220.1614290919740401No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG385820.16043112736444323No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG381760.15874290389987522No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA381310.1585557855355758No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA379300.15771999017503843No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA376600.15659727998924194No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA364250.15146192043569137No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG350870.14589826773718886No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA341360.14194383297166127No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA339130.14101655752191083No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG329790.13713281191622967No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG323390.1344715729573047No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA320100.13310352980498233No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG318270.13234258179016473No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA314280.13068346562670993No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG302540.1258017554114319No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG298640.124180062920837No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG295790.12299497994694072No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA278060.11562251639354384No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG277670.11546034714448436No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA271360.11283653185841926No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG267770.11134374312989728No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA266290.11072833162064587No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGAGATCGGAA260500.10832074199999342No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG259670.10797561257250785No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG257500.10707328623799735No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATC256420.10662420216367877No Hit
GGGGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAA254680.10590067782172102No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA248260.1032311224910494No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA247180.10278203841673081No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG246960.10269055832751775No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG245280.10199198310079996No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)