FastQCFastQC Report
Fri 14 Jul 2023
SRR9132360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19887018
Total Bases795.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG11739275.902981532977946No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA9922024.9891944584150325No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA5502052.7666541057085583No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4722102.374463582222332No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG4610082.3181353785670633No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3427511.7234911739909924No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2072601.0421874209597437No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1978970.9951064558799112No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1671640.8405684552606127No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA1631980.8206257971909112No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1623050.8161354306613491No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1529840.7692656586321791No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1398060.7030013247838364No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1329390.6684712610005181No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA1252150.6296318533024912No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1233690.6203494158852775No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG1215910.6114089100738984No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1202900.6048669539093292No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1092830.5495192894178504No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG1004860.5052844021159935No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1001070.5033786362540628No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG988960.4972892366266275No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA975980.4907623656799627No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA948980.477185669565945No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA938950.4721421783798858No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG842660.4237236573125242No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA805030.404801765654358No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA694120.3490317150615542No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC692850.3483931075035986No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA687090.34549674566594146No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG671210.3375116369885118No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC642410.32302982780022627No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA636440.3200278694372379Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA620230.31187682336285916No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA593610.2984912066756313No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG579440.2913659554187561No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA570160.28669959468030853No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA553160.27815130453444553No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC536820.26993488918248076Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA521180.2620704622482868No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG512100.2575046696292023No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA510740.2568208064175333No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG494490.2486496467192819No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG491070.246929931878173No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA480170.24144896937288435No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA477700.24020695309875015No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG459640.23112565192026277No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA453470.2280231254379113No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG426490.21445648613582993No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC417370.20987057989287286No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG413390.20786927431754726No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC407230.20477177624116397Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA402990.20263973211066635No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG384240.1932114709203763No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA383970.19307570395923612No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG382540.19235664190579No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG380120.19113976766149657No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG379640.19089840417502513No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA374540.18833391713126624No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA357250.17963980321232675No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG346980.1744756302830319Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG345280.1736208012684456No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG342570.17225810325107566No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA335450.1686778782017495No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA309650.15570459080391036No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA305760.1537485408822982No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG293350.1475082890758182No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA287310.14447113187105276No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG273910.13773306787372547No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA273230.13739113626789096No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG273180.13736599423805018No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA270090.13581221679389036No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA267370.13444449037055228No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG264710.13310693438302312No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA262540.13201577028793357No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAA261170.13132687867029635No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG260550.13101511750027078No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAGATCGGAAG260010.13074358357799043No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG254250.1278472217403333No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA254140.12779190927468362No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG253950.1276963695612887No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGAGATCGGAA251450.12643926806925No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA247050.12422676944326193No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA241640.12150640181449024No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTAGATCGGAA239800.12058117511634978No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG239260.12030964119406941No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGA236640.11899219883041288No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA235600.11846924460972479No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAA234490.11791109154725964No Hit
CGCCGCGGCCCGGGTTCGGTTCCCGGTCAGGGAGATCGGA227620.11445657664713735No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA224440.11285754354926414No Hit
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA220940.11109760146041No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAAGATCGGAA220280.11076572666651179No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTAGATCGGA219690.11046905071439067No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA218600.1099209544638618No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGA214180.10769839902593742No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCG212080.10664243377262493No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAG211070.10613456476984132No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGG205300.10323317452621604No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)