FastQCFastQC Report
Fri 14 Jul 2023
SRR9132361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19852589
Total Bases794.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG11318795.701417583369102No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA9629324.850410190832037No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA5460822.75068405435684No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG4727122.3811100909810805No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4685802.360296684729634No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3411071.718199072171393No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC1930750.9725431781215035No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1872030.942965171948102No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1652220.8322440967271323No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA1602600.8072498755703853No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1517420.7643436329639425No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1412550.7115192884917931No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1351780.6809086714080466No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1246410.627832470616301No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1184900.5968491061795517No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG1181520.59514655745908No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1149560.579047901510478No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA1124530.5664399741514823No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1077250.5426244405704465No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1022520.5150562478274244No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1004940.506200979630415No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG987670.4975018623515553No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA947610.47732313402549154No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA921470.46415608563699173No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA920490.46366244725058275No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG813650.40984578887922374No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA764150.38491201323918006No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA678800.34192013948407435No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC676630.34082708305702597No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA641180.3229704699976411Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG639500.3221242327637972No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA627460.31605953258791586No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA621160.3128861429610012No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC612070.3083073950707387No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA601850.3031594518981882No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG569370.28679886537720595No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA557390.2807643879596762No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA540560.2722869042420613No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC519620.26173916157736404Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG518240.26104403813527793No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA510540.2571654508134934No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA507610.2556895727806585No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG502760.25324656648057337No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG489670.2466529680335396No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA486410.24501086482977105No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA461250.23233745482768015No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA428800.21599197968587372No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA413200.208134062514466No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG402870.20293071095160437No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC392910.19791373306524404Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG389840.19636733526292213No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC389450.19617088733363694No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA380460.19164251070729366No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG377940.19037315485652778No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG368040.18538639972851903No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG364510.18360829411216845No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA363020.18285776227977116No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA358870.18076735482712103No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG358780.18072202068959367No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG346750.17466235764010427No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG344650.17360456109779937Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG344170.17336277903098685No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG332760.16761541781779696No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA326010.16421535750324553No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA305850.15406051069711865No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA305840.15405547357072671No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA280400.14124102402966182No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG276540.1392966932423776No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA270650.136329825797532No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA264670.13331762421515903No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG257090.12949948241007758No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG255570.12873383919850453No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG255250.12857265115396285No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA251650.12675928565286876No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG248710.1252783704936419No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGAGATCGGAA248700.12527333336724997No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA247870.12485525187671996No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA244550.12318292591459985No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG241020.12140482029824927No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG239590.12068451122420355No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA237170.11946552663735696No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGA235700.11872506905774355No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTAGATCGGAA234600.11817098515463148No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG234040.1178889060766835No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAA233740.11773779228492565No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA233390.11756149286120818No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAA231120.11641806517024053No Hit
CGCCGCGGCCCGGGTTCGGTTCCCGGTCAGGGAGATCGGA225930.11380379657282987No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA221840.11174361187853131No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAG216820.10921497442978345No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA215480.1085399994932651No Hit
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA212880.1072303466313638No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTAGATCGGA209220.10538675837191815No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGG205750.10363887551391912No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCG204310.10291352931348148No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA202740.10212270046994877No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)