FastQCFastQC Report
Fri 14 Jul 2023
SRR9132362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23233587
Total Bases929.3 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG13021415.604562911443678No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA10396074.474586726535167No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA7513123.233732268719419No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG7180353.0905042772775464No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4775782.055550010422411No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA3661061.575761848568626No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3303301.4217778770019456No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2288290.9849060328050077No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2239810.9640396896097017No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1718660.7397308043738575No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1623950.6989665435647109No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1474670.6347147343197588No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1308530.5632061893843598No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1283600.5524760339417241No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1283210.5523081735076035No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1173630.5051436956333949No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1136170.48902048573042123No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA1130040.48638206403514017No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG1100980.47387430963630367No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA926600.39881917501589403No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA900450.3875639177024194No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC882060.37964865261657615Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA854640.36784677286378553Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC847550.3647951562537459No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA833440.35872205182953454No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG831780.35800756895609787No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA709360.30531660909699393No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA684120.2944530261298008No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG669470.28814749956603775No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA658460.2834086703874008No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA655550.28215617330203896No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA631070.2716197029756963No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG624400.2687488591408636No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG590600.25420095485040684No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA580700.2499398822919595No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG551740.237475169030077No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA547540.23566744127800845No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC544860.23451393880764088Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG535920.23066606116395202No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA502580.21631614610348374No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC484090.2083578398806865No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA478360.2058915827332215No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG472380.20331772274337148No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA470850.20265919334797508No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA468490.20164342251586032No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG453280.1950968655851548No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG449980.19367650806567233No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG432010.18594201575503602No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC417300.17961066450910057No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA410530.1766967795373138No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA407570.175422761883475No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA405180.17439407871027404No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG402690.1733223544001191No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA398740.17162222949043557No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAA397620.17114016875655058No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG395200.17009857324226346No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGAGATCGGAA370700.15955349468853003No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG360500.15516329871922058No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA358970.15450476932382418No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA355880.15317479819194513No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG355000.1527960361867498No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA350340.15079031920469274No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA346350.14907297784022758No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGA329620.14187219562782105No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA327970.14116201686807983No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG321710.13846764169475856Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA320030.1377445505939311No Hit
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA319200.1373873091572128No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG319190.1373830050435174No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA317390.13660826457834513No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG314910.13554084438188557No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA314100.13519221117255809No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG309130.13305306666594358No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA299680.12898567922378926No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG294080.12657537555436446No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA291650.12552947592638192No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG289080.12442331870666376No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA288410.12413494308907186No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAGATCGGAAG286490.12330855325955481No Hit
CACCCGCCCTCCCTTCCCCCGCCGAGATCGGAAGAGCACA285900.12305461055152611No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA283900.12219378781244583No Hit
TCCCCCGCCCCACCCCGCGAGATCGGAAGAGCACACGTCT281860.12131574861858395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA270760.11653818241668838No Hit
GGGGGGAGAAGGGTCGGGGCAGATCGGAAGAGCACACGTC259630.11174770387370662No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA259410.1116530133724078No Hit
CGGCGGCGGGGGCAGATCGGAAGAGCACACGTCTGAACTC244950.10542926496885736Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGAGATCGGAA243760.1049170754391046No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAG237910.10239916892729478No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG235400.10131883638974902No Hit
GGGGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAA235050.10116819241040999No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGAGATCGGAAG234430.10090133736129508No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)