FastQCFastQC Report
Fri 14 Jul 2023
SRR9132363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23516479
Total Bases940.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG12725425.411277768240731No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA10244194.356175088966338No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA7539963.206245288676081No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG7388433.1418096220952125No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4788742.0363337555762495No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA3647481.5510315128382952No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3331331.4165938701962995No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2237050.9512691079306558No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2156060.9168294284191099No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1673220.7115095759020728No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1601630.681067093419895No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1447050.6153344639731144No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1260840.5361516917562361No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1247880.5306406626604264No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1213400.5159786037697225No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1198040.5094470137302443No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1116680.47484999773988273No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG1088120.4627053225102278No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA1084480.46115747174566396No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA947220.40278989044235747No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA901860.3835012886070232No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC861420.3663048367062093Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA848330.3607385272259508Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC839900.35715380691131526No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA827660.351948946098606No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG793840.337567541467411No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG680200.2892439807847085No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA675710.2873346813525953No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA633960.26958117327002906No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA626240.2662983689012288No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA613670.26095318095876513No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA593700.2524612634399903No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG583310.24804308502135883No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA579030.2462230846718167No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA559840.2380628494597342No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG544620.2315907921419699No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC536390.22809111857264006Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG524060.22284798672454323No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG522130.22202728563234317No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA508710.21632064902233025No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG488070.20754382490678133No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA479120.20373798305435095No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC461350.1961815797339389No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG460560.19584564508998134No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA456110.1939533550069294No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG444750.18912269987356525No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA442010.18795755946287707No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG424910.18068606273923915No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA414230.17614456653991442No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA408800.17383554740486448No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG392390.16685746195253126No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA390330.16598148047588246No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA390040.1658581626951892No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC386120.16419124648719732No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGAGATCGGAA384110.16333652669687498No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG379140.16122311507602818No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA368680.1567751702965397No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG363000.15435984272985764No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA357440.151995543210359No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG353340.150252084931592No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAA353210.1501968045471433No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA349850.14876801922600744No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA346870.1475008227209524No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA335090.14249156942244628No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA318730.13553474565643947No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG316370.13453119406183214Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA316130.13442913796746528No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA311940.13264740865331073No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGA307020.13055525871879034No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA302680.12870974434565652No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA301960.12840357606255598No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG298070.12674941686635996No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA295800.1257841363071402No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA292240.12427030424069861No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG291490.12395137894580222No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG289730.12320296758711201No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA288820.12281600489597103No Hit
CACCCGCCCTCCCTTCCCCCGCCGAGATCGGAAGAGCACA284750.1210853036289999No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG277620.11805338715885146No Hit
TCCCCCGCCCCACCCCGCGAGATCGGAAGAGCACACGTCT276090.1174027795572628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG267450.11372876016005629No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA256160.10892787138754913No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGAGATCGGAA238370.10136296339260652No Hit
GCAGCAACCTCGGTTCGTATCCGAGTCACGGAGATCGGAA236540.1005847856730593No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)