FastQCFastQC Report
Fri 14 Jul 2023
SRR9132364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20389134
Total Bases815.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG10617385.207371730452112No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA8123103.984033848617602No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA6011172.9482223227332756No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG5055212.4793647439856934No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC3789551.8586125335190793No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA3643761.7871087609704266No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA2965311.4543579928406964No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA2526791.2392826492777966No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2472461.212636103132188No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG2138311.0487497899616531No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1717570.8423947775319932No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1688980.8283726027795001No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1323390.6490663114970945No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1214360.5955917499978175No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA1162300.5700585419665201No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA1162220.5700193053809937No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1100780.5398856076967271No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1076090.5277762164886454No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC1074550.5270209122172624No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA1068190.5239016036679145No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG1023620.5020419209565251No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1010180.4954501745880919No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA906220.44446223169654975Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA899360.44109769448766195No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG869060.4262368377195421No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG863390.4234559447203594No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG778840.3819877783921573No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA775430.38031531893409504No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC755800.3706876417605574No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA742650.3642381280146572No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC729520.3577984234151387Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA716460.3513930508279557No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA626190.30711946863461687No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG617640.3029260585564841No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA610650.2994977618961159No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG581110.28500965269049683No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC551460.27046759317977903No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA545400.26749542182615504No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA531220.26054073704160263No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG512120.2511730022471773No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC503550.24696978302266295Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA479890.23536556285323348No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG473650.23230510918217517Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA473190.23207949881539847No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG451690.22153466645518147No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA429410.21060727738608223No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG401200.1967714764148394No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG389870.1912145949896646No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG388660.19062114163357796No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA384880.1887672129674561No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA384770.1887132626623573No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGA383840.18825713735561306No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA378360.1855694312470554No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG361770.17743274432352055No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA361200.17715318365164504No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG360040.1765842531615124No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG350370.17184153088601017No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG348210.17078214307679765No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG324450.15912887717546023No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG324260.15903569028483505No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA319970.15693162838598246No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA314140.15407226221574688No Hit
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA313960.1539839798983125No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA312760.15339543111541667No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA300530.14739713810307No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG276580.13565068531110738No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA270060.1324529035907067No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA267000.13095210419432232No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA265370.13015265876422216No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA263040.12900989321076609No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG249730.12248190629381317No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG247130.12120671726420552No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG242880.11912227365811613No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTAGATCGGAA238260.11685636084396718No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA237610.11653756358656528No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA235070.11529180199610244No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA234850.11518390138590487No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAGATCGGAAG231320.11345258704955295No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG228600.11211854314165574No Hit
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGT225090.11039703795168544No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG212540.10424179859723322No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG211910.10393281048621289No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA209150.1025791482855525No Hit
GGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACG207050.1015491879154848No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAG206080.10107344431597733No Hit
GGGCCGCCGGTGAAATACCACTACTCTAGATCGGAAGAGC205880.10097535285216136No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)