FastQCFastQC Report
Fri 14 Jul 2023
SRR9132365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20561915
Total Bases822.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG10416945.066133188470043No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA8019883.900356557256462No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA6027702.9314876556974387No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG5204882.5313206479065786No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA3663641.7817601132968401No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC3585491.7437529529715496No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA2951151.4352505591040523No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA2552381.2413143425600193No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2323111.1298120821917608No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG2005440.9753177172456943No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1687520.8207017682934689No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1645940.8004799163891108No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1255010.6103565742782227No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1194050.580709530216422No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1130170.5496423849626847No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA1111060.5403485035318938No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1048890.5101129928802838No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA1048800.5100692226380665No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC1032310.5020495415918216No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA1020070.4960967886502789No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG1003410.48799443047984586No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG988220.4806069862656275No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA910250.4426873664247712Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG889700.4326931611185048No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA882380.4291331814181705No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG856860.4167218860694638No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC759430.3693381671891942No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG752150.3657976409298453No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA749490.364503987104314No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA744820.36223279786926466No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA715090.34777402785684114No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC707280.3439757435044352Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA639010.3107735831025466No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG610910.2971075408102796No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA556200.2705000969024529No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG552400.2686520200088367No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA542860.26401237433381086No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA517490.2516740293887996No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC516150.25102233911578764No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC499450.2429005275043691Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA480140.23350937886865109No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG470890.22901077064076958Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG467860.227537172486123No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA453360.22048530012890336No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG440560.21426019901356463No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA418510.2035364896703444No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG394780.191995735805736No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA385720.18758953142253532No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG372100.1809656347669952No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA365790.17789685445154305No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA361390.17575697594314538No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG358770.17448277555859948No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGA355320.17280491627360584No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG347400.16895313495849001No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG342330.1664874113135863No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA335950.16338458747640966No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG334430.16264535671896319No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG330590.16077782638436158No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG328630.15982460777607532No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG328080.1595571229625256No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA325570.15833641954068967No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA325130.1581224316898499No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA313520.15247607044382783No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA305670.14865833265043651No Hit
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA301230.14649900070105337No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG274760.13362568612894277No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA266770.1297398612921024No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA265790.1292632519879593No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA265650.12919516494451028No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA248160.12068914787362948No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG244440.11887997786198415No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA244050.11869030681237619No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA241980.11768359124137999No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA240650.11703676432861435No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG239680.11656501838471756No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG238930.1162002663662407No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTAGATCGGAA236020.11478502853455042No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA220670.1073197705563903No Hit
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGT214700.10441634448931435No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG206100.10023385467744614No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)