FastQCFastQC Report
Fri 14 Jul 2023
SRR9132366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23632216
Total Bases945.2 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA13412165.675371281305147No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA9197973.8921318254707895No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG8477153.58711599453898No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG8154243.450476248185951No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4973572.104571996125966No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA4817972.0387296730869418No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA4659381.971622128030651No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2861311.210766692382974No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2618471.1080086607197563No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2436881.0311686386075687No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1983390.8392738116476254No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1583960.6702545372808034No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1528960.6469812225819195No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1511810.6397241799076312No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG1442450.6103744143164568No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1220830.5165956506152449No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1207390.5109084988051903No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1204960.509880241446676No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1135830.48062780062606064No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG968060.4096357277709377No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG943490.39923890336818185No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA840830.3557982036047741No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC764710.32358793606151875No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA725520.30700464146062306No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA708360.2997433672745713No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC696580.2947586464172467No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA695380.2942508650056347No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA656490.277794515757642No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA615010.2602422049629201No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA604020.25559177353490675No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA597200.2527058825122452No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA589030.24924873740152No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG588760.2491344865839073No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG587630.24865632575463933No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA580810.24577043473197774No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC572800.24238099380946757No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA551940.2335540602709454No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA524510.22194702350384746No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG521540.22069026451010773No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA512080.2166872543818997No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG505040.21370827010044252No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA476010.20142419145119528No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA435240.18417231799167713No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG431470.1825770380568627No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG425150.17990272262237278No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCG420640.1779943108170643No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG419930.17769387348186053No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG419130.17735535254078585No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC410280.17361046463014723Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA408240.17274723623040683No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA407330.1723621686599344No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG404070.17098269582505507No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA403360.1706822584898513No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG398830.16876538366101596No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA389480.1648089201622057No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA389250.16471159539164673No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA384470.16268893276872554No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA370090.15660401885290826No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA368910.1561047004648231No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA363860.15396778702428923No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA360280.15245290581298004No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG354930.15018904701954314No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA352110.14899576070225493No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG328850.13915326434050876No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG316210.13380463347152888No Hit
GGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCAAGATCGG315330.13343226043634673No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG311500.13181159143095172No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG307310.1300385880020731No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG305950.12946310240224615No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA304960.12904418273766624No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG301310.12749968094401304No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG292530.12378441361571849No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA291920.12352629139814902No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAAGATCGGAA291340.1232808637158699No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG284010.12017916559327317No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA283700.12004798872860675No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAA278900.1180168630821587No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAAGATCGGA271480.11487708135369107No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG267590.11323102327771546No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA266400.11272747337786689No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA260100.11006162096690382No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGA260020.11002776887279635No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA255860.10826745997920804No Hit
GGCCGTGATCNTATAGTGGTTAGTACTCTGCAGATCGGAA247250.10462412835089184No Hit
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA246110.10414173600986043No Hit
GGGGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAA243400.10299499632196998No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA242720.10270725352205651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)