FastQCFastQC Report
Fri 14 Jul 2023
SRR9132367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23874350
Total Bases954.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA13092805.484044591789933No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA9157313.8356269385344524No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG8381373.510617042977086No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG8210443.439021376498208No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4975262.0839352694418904No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA4829372.022827846622002No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA4635421.9415900328176476No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2687861.1258358866314684No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2587151.0836525392314345No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2258660.9460613587385626No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1851820.7756525308542431No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1552720.6503716331544105No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1550520.6494501420981095No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1444970.6052395143742133No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG1389000.5817959441827736No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1184640.4961978022438307No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1183940.49590460054409863No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1160040.48589385679610125No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1148750.4811649322389929No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG934950.39161275594937667No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG923850.386963414710767No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA858640.35964958208286296No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC713190.29872645747423493No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA696540.2917524456163204No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC687260.2878654287970144No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA687120.287806788457068No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA653990.27392997086831683No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA647210.27109010297662556No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA601850.2520906328339829No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA594290.24892405447687582No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA593360.2485345150758031No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA586600.24570302437553274No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA563470.23601480249724077No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG555080.23250057069616556No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA554200.23213197427364515No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC540190.2262637516832919No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA527200.22082276585540547No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG524040.2194991696109004No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA510400.21378592506183414No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG501710.21014603538944515No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG501330.2099868687524477No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA443890.18592757499157045No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG440900.18467518487414317No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCG426930.17882371666663177No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG418000.17508330069719177No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA414010.17341205100871857No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC401900.16833966160335256Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA399630.16738885037707832No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA394960.16543277618029392No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA391110.16382016683176714No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG385280.16137821553256948No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA385190.16134051817117534No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG384570.16108082523712686No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA380050.15918757997599933No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG376510.15770481709449682No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG375970.157478632926132No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA372730.15612152791594328No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA369510.15477280009717542No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA360160.15085646310789613No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA359020.15037896319690378No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA356280.14923128797223797No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG353400.14802497240762577No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA325070.13615868075989504No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG317340.1329208962757101No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG311080.1302988353609627No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA310460.13003914242691422No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG307710.12888727860653798No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG307360.12874067775667192No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG300630.1259217528435329No Hit
GGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCAAGATCGG287230.12030903459151766No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG285440.11955927595934548No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA281300.1178251973352154No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAA280440.11746497810411594No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG272750.11424394800277286No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAAGATCGGA269200.11275699652555987No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG268420.11243028606014403No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA266490.11162188708802544No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA264130.11063337850035708No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG263820.11050353203333285No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGA259990.108899299876227No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA259960.10888673408909562No Hit
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA244990.10261640631053828No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA244640.10246980546067222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)