FastQCFastQC Report
Fri 14 Jul 2023
SRR9132368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23323418
Total Bases932.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA13004635.575782245981271No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG9242793.962879711712923No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG8991233.855022449968525No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA7468373.2020907055732564No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA5059472.1692660998486586No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA4466991.9152381524869124No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA3191821.3685043933097627No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2330200.9990816954873423No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1987120.8519849020413731No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1904190.8164283639730677No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1890050.8103657877245951No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1819920.7802972960481178No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1662300.7127171497762463No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1524180.6534976991794256No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1297720.5564021534065033No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1277500.5477327551219122No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG1252830.5371554032089122No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1216420.5215444837459072No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG1183340.5073613138520263No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1082130.46396715953039125No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA781440.33504523222110927No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA767820.3292056078573046No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA709300.30411494575966525No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG698740.29958730748640705No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA679150.29118802398516375No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA673100.28859406455777625No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC612820.2627487960812605Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA600710.2575565896902418No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG594130.25473539084194263No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA557350.2389658325379239No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA556050.23840845282625386No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG548290.23508132470120802No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA540850.23189139773595793No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA534350.22910449917760764No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG534050.2289758730902992No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA523670.22452541046942606No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA521060.22340636350984236No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC520860.22332061278497No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC513430.22013497335596355No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA506090.21698792175314954No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC504870.21646484233142846No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA491340.21066380579381636No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA483930.20748674143729706No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA471360.20209730837907205No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG465240.19947333619797925No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA464530.19916892112468249No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA459620.19706374082906716No Hit
GGGGGGGATGCGTGCATTTAGATCGGAAGAGCACACGTCT422780.18126845730758673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG414010.17750828802193572No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA403190.17286917380634348No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA395240.16946058249266896Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGGGGAGAAGGGTCGGGGCGGAGATCGGAAGAGCACACG389610.16704669958751328No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG381000.16335513088176012No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG378600.16232612218329234No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG369760.15853594014393602No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG365300.1566236989792834No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA360730.15466429491595102No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG356680.15292784273728663No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC343560.14730259518566274Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA335670.14391972908944992No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA331250.14202463806977178No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG329100.14110281777739436No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCG328630.14090130357394443No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG324340.13906195052543327No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA323370.13864605950980255No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA322150.13812298008808144No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA319640.13704680849093387No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA315100.13510026703633232No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG306830.1315544745628621No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG305450.13096279456124313No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG305080.13080415572022935No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG302300.12961222064450417No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG299930.12859607455476724No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA297680.12763137889995368No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG297190.12742128962401653No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA294400.12622506701204772No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA292620.1254618855606841No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAAGATCGGAA287160.12312089077166992No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA284830.12212189482690744No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG274130.11753423104623859No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA273130.11710547742187702No Hit
GGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCAAGATCGG269030.11534758756199456No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG265490.11382979973175457No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA264790.11352967219470148No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGA259500.11126156552182875No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG258130.11067417305645338No Hit
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG254530.10913066000875173No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG249690.10705549246684169Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAAGATCGGA233700.10019972201330012No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)