FastQCFastQC Report
Fri 14 Jul 2023
SRR9132369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23563303
Total Bases942.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA12733885.404115034297187No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG9516824.038831058616867No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG8744623.711118089004754No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA7464153.1677010646597377No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA5050252.1432691333638583No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA4490041.9055223285122633No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA3169911.345274047530603No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2183460.9266357946506906No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1902150.8072510038172492No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA1885580.800218882726246No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1862490.7904197471806054No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1768290.7504423297531759No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1563730.6636293731825288No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1497630.6355772787881224No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1243920.5279056166276859No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG1227290.5208480322134804No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1206410.5119867957391203No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1180670.5010630300853832No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG1137130.48258514521499807No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1107750.4701166046203285No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA779080.330632764005963No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA761350.3231083519997175No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA687990.29197519549784684No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA682230.2895307164704371No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG664560.2820317677873938No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA645470.2739301871218988No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA610230.2589747286278159No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC599900.2545907931498398Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG585550.24850081501731738No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA561120.238132998586828No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA540990.22959005365249516No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG539280.22886434894123292No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA524270.22249427425348645No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA520880.221055596492563No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA500730.21250416378382947No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA499770.2120967506125945No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA497290.21104426658690423No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC496850.21085753555008818No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA494970.21005968475641978No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA494690.20994085591480954No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC493870.20959285716437973No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC482600.2048099962895694No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG467470.19838899495541862No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA452430.19200618860607105No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG451510.19161575098363756No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA447790.19003702494510213No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA432830.18368816969335752No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA408370.17330762160126703No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA394420.16738739895675916Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGGGGGATGCGTGCATTTAGATCGGAAGAGCACACGTCT389270.16520179704857169Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG380130.16132288414743892No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG376190.15965079259049547No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA374360.15887416123282885No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG374100.15876382016561938No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG362980.15404461759881458No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGG361290.15332740066195302No Hit
GGGGGGAGAAGGGTCGGGGCGGAGATCGGAAGAGCACACG355120.15070892225932842No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG346670.1471228375750208No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCG342550.1453743560484708No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC329770.13995066820640553Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG323860.13744253087099037No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA322880.1370266299253547No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA321750.13654707067171354No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA321430.1364112662813019No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG319410.13555400106682836No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG314220.13335142360983943No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA310760.13188303863851347No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA305700.12973563171512922No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA299870.127261445477317No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG295100.12523711128274334No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA293380.12450716268428072No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG290260.12318306987776713No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA288780.12255497457211324No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG286330.12151522220802406No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG282290.11980069177907697No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA282000.11967761905026643No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG275920.11709733563244507No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG271760.11533187855709363No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA269880.11453402776342518No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG265950.11286618009368211No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA265900.11284496065768031No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGA263160.11168213556478053No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAG258540.10972145967821235No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA258460.10968750858060944No Hit
GGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCAAGATCGG252680.10723454177879901No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG246980.10481552607459149Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CAGGAGATCCTGGGTTCGGATCCCAGCGGGGCCAGATCGG244520.10377152982330193No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)