FastQCFastQC Report
Fri 14 Jul 2023
SRR9132370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21519343
Total Bases860.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG13426526.239279702916581No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA11441915.317035004274992No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG5827232.7079033035534588No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA5788292.68980795556816No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4731362.1986544849440803No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3446411.6015405303033647No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC3303341.5350561585453608No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2349031.0915900174089888No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA1844320.8570521878851042No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1754760.8154338169153212No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1632820.7587685181652618No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA1487370.6911781646865335No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1333340.6196007006347731No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC1315620.6113662484955976No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1313800.6105204977679848No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1285270.5972626580653508No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1168620.5430556128037923No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1101390.5118139526843362No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG1031680.4794198410239569No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA993610.46172878047438526No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG969780.45065502232108107No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG891000.41404609796869724No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA879290.4086044820234521No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA827640.38460281989092326No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC773380.35938829545121337No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG765080.3555313003747373No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA712110.3309162366155881No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA705610.32789569830268517No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC667020.30996299468808136Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA623070.2895395087108375No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG620880.28852181964849016No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA615090.28583121705899667Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC611610.2842140673160886No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA604420.2808728872438159No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG556260.258493021836215No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA531950.24719620854595795No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA526990.24489130546411197No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA517820.24063002295190888No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA490520.2279437620377165No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA475790.22109875752247637No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA474310.2204110041835385No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA472180.21942119701331028No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA453700.2108335742406262No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG452760.21039675793076024No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG443280.20599141897594178No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA427810.19880253779123275No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA425280.19762685134021052No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC420360.195340536186444Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA380420.1767804899991603No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG373950.1737738926323169No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA371970.17285379019238645No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA355990.16542791292466502No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA346350.16094822225752894No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG320580.14897294959237373No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA312260.14510666055185792No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG307190.1427506406677936No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG299640.13924216924280636No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG296700.13787595652897025No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG296150.13762037251787845No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG293040.13617516110970487No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG292770.13604969259516891No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG289500.1345301294746777No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAGATCGGAAG288810.13420948771530805No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG284260.13209511089627596No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG283020.13151888512581447No Hit
GGGGGGAGAAGGGTCGGGGCGGAGATCGGAAGAGCACACG273450.12707172333281735No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA269440.12520828354285723No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG269180.12508746201034113Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG267380.12425100524676799No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA267260.12419524146252978No Hit
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA264290.12281508780263412No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA262120.12180669270432651No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAA257210.11952502453257982No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG256730.11930196939562698No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAA256660.11926944052148804No Hit
GGCCGTGATCGTATAGTGGTTAGTACAGATCGGAAGAGCA255660.11880474231950296No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA250930.1166067198241136No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA250680.11649054527361731No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAAGATCGGAA248920.1156726764381236No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG248140.11531021184057524No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAAGATCGGA241420.11218743992323557No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA240920.11195509082224304No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGAGATCGGAAG240690.11184821023578646No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGAGATCGGAA237250.11024964842095783No Hit
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA235860.1096037179201986No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG229180.1064995339309383No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA228020.10596048401663564No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA220360.10240089578943No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTAGATCGGAA216210.10047239825119196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)