FastQCFastQC Report
Fri 14 Jul 2023
SRR9132371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21604846
Total Bases864.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG13043756.037418642095389No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA11196195.182258646972073No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG5950642.7543079918273894No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA5761412.6667211606136885No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA4681242.16675462532804No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA3421961.583885393119673No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC3075131.4233519646471908No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA2167611.003298056371242No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA1833870.8488234537751391No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG1755120.8123732981017314No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1595130.7383204675469568No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA1421400.6579079526880219No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC1296630.6001570203277543No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1293950.5989165578870592No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1284840.5946999113069354No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1242710.5751996565955619No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1186440.5491545739321633No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG1018500.47142201337607315No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG1012150.46848285796621736No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG930320.43060709620424975No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA928790.42989892175116634No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG867120.4013543998415911No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA826900.38273820604877257No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA778900.3605209683049812No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG777090.3596831932983924No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC732730.33915076275017186No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA661820.30632942257491674No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC643760.29797018687381527Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA631120.2921196476012835No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA621470.2876530570965421No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA610900.28276063620171143Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG603900.2795206223640752No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA574700.2660051360699354No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC566950.26241797789255245No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG528700.2447136165654687No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA523860.24247337842630304No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA516680.2391500499471276No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA514960.23835393226130844No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA479450.2219178049220994No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA462660.21414640030296908No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG458790.2123551355098759No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA457280.2116562182391858No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG448570.20762471530692697No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA442300.20472258862664425No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA433590.20069108569438543No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA426130.1972381566617045No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC403370.1867034830981901Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA387870.17952916674342415No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA380170.17596515152202427No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA368650.17063301446351437No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA358050.16572670779509374No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA349580.1618062910515539No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG319300.14779091690817883No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG313660.14518039147328335No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA312110.14446295983780677No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG303350.14040831394956482No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG300050.13888087885467917No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG290460.13444205989711752No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG286810.13275262411035005No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG283970.13143810421050908No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAAG283100.13103541677640285No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG277090.1282536334672323No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA268050.12406938702548492No Hit
GCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCAG263650.12203280689897072Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA259610.12016285605553495No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAA257080.11899182248278928No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG256920.1189177650236433No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAA256770.11884833615569396No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG252330.11679324166439327No Hit
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA252020.11664975533729795No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG250050.11573792287156318No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA249980.11570552273318681No Hit
GGCCGTGATCGTATAGTGGTTAGTACAGATCGGAAGAGCA249220.11535374980224343No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG247210.11442340297172218No Hit
GGGGGGAGAAGGGTCGGGGCGGAGATCGGAAGAGCACACG245590.11367357119786922No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA245310.11354397064436378No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA242470.11222945074452279No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGAGATCGGAA240610.11136853278195086No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA235970.10922086646671769No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAAGATCGGA235560.10903109422765614No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGAGATCGGAAG232710.10771194573661853No Hit
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA230930.10688805650361961No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG227510.10530507831437448No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA218360.10106991736946423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)