FastQCFastQC Report
Fri 14 Jul 2023
SRR9132372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23410660
Total Bases936.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG12334985.268958670964423No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA12241705.2291135747561155No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA7864243.3592559970543334No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG6016322.5699061880357066No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA5048822.1566329185080644No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA4886312.0872158239024445No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA4003411.7100799379428002No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG3256161.3908877408838538No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2326370.9937225178615212No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2269910.9696052994661407No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1892460.8083753298710928No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1559800.6662776700870459No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1400530.5982445603840302No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1292230.5519835835469824No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC1287940.5501510850185343No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG1238750.5291392895373304No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1177420.5029418222297022No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1077640.46032021309950255No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1013460.43290535166458355No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG999530.42695507089505375No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA951290.40634907345628013No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG932460.39830572909947864No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA920410.3931585012981266No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG905550.3868109656028493No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG870590.37187759764141637No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA813530.3475040857455535No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA807060.3447403874986865No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA756580.32317756099144573No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC718020.3067064320271193Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG671850.28698464716500943No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG640420.27355913929808046No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA635530.27147034726915004No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA608500.2599243250724243No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC595850.25452080377059No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA580380.24791270301648907No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA568620.2428893504070368No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA568480.2428295485902576No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA566220.24186417640510777No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA564360.24106966655361275Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC540150.2307282238091536No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG540060.23068977978408126No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA539130.2302925248583338No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA531890.22719991661918118No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG526110.22473095589786873No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA523440.2235904498207227No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA511380.2184389504610293No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA497960.2127065191669094No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG490030.20931917340220224No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA487330.2081658526500321No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA485250.2072773685150269No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG472350.20176705825465835No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC471480.20139543267895907Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA465350.19877696741569867No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA440980.18836717973777758No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA440560.18818777428744No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG383800.1639424091418183No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG363750.1553779346673695No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG357480.1526996675873299No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAA344870.14731323251886108No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG343760.1468390895429689No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG342810.14643329150053863No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA339130.14486135803091413No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG337960.14436158570497373No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA337900.1443359563549255No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG318870.13620718083129651No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA317510.13562624889687006No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA315210.13464379047835473No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA313270.13381510816012876No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG296140.1264979287213603No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG282750.12077831210226453No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG280740.11991972887564896No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAGATCGGAAG270720.11563962741759524No Hit
GGGAGACCGGNGTTCGATTCCCCGACGGGGAAGATCGGAA270080.11536624768374748No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA267310.11418302602318772No Hit
GGGGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAA263630.11261109255356322No Hit
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA261830.11184221205211642No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA261580.11173542309358216No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG258850.11056928766638788No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGG258030.11021901988239545No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAGATC257320.10991573924015811No Hit
GGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCAAGATCGG256090.10939033756416948No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA255010.10892900926330143No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG254830.10885212121315674No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA254030.10851039654584706No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAAGATCGGA252540.10787393435298279No Hit
GGCCGTGATCGTATAGTGGTTAGTACAGATCGGAAGAGCA249300.10648994945037858No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG248780.1062678284166273No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA248440.10612259543302069No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA247660.10578941388239375No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGAGATCGGAAG240000.10251740019290358No Hit
GGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCA237270.1013512647657093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)