FastQCFastQC Report
Fri 14 Jul 2023
SRR9132373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9132373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23644362
Total Bases945.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG12007345.078310000498216No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAA11999075.074812337926479No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA7848123.319235257859781No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAG6193172.6193009563971317No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAA5061152.140531429860531No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA4867092.058456895559288No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGA4039771.7085552995678206No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCG3036321.2841623724082722No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAG2304790.9747736056485686No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCAC2140540.9053067280901892No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCA1768480.747949976404523No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAAG1539720.651199639051373No Hit
GGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGAA1377730.5826885918934924No Hit
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGC1270460.537320482574239No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAG1264670.5348716958402177No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGG1207220.5105741487124923No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA1192800.5044754432367429No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGA1041070.4403036969236049No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAG989990.41870023813710855No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA957170.4048195506395986No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG922350.39009299553102766No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAG921440.3897081257679949No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGA905310.38288620348478847No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGA886510.37493504794081567No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGG851140.359975879239203No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA823110.34812104467018395No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCAGATCGGAA757940.3205584485637633No Hit
GGAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTC710280.3004014233921812Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAA682530.2886650102887107No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGG648390.2742260501679005No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAA637010.2694130634609638No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAGATCGGAA583340.24671420611814351No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGG582310.24627858429844712No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAGATCGGAA575380.243347653026121No Hit
GGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAGATCGGA565880.23932978187358153No Hit
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGC562810.238031375090603No Hit
AGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCCA558600.23625082376931972Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA557400.23574330320268316No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGA557070.23560373504685808No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAG554010.2343095576019349No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAA532810.22534336092468896No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGA526420.22264081390734924No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCAC514510.21760367228348138No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGGA501150.21195327664159433No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGGGCCAGATCGG499530.21126812387663496No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACA488780.20672158546718242No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAA488460.20658624664941266No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG474400.20063979734365428No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGA469420.19853358699211254No Hit
GAGCCTCGGTTGGCCTCGGAAGAGCACACGTCTGAACTCC462090.1954334821975742Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGA451460.19093769584478532No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGA449570.19013835095233272No Hit
CGGGAGACCGGGGTTCGTTTCCCCGACGGGGAAGATCGGA446710.1889287602685156No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGA436720.18470365155126622No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGG421550.17828774572136902No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGG375300.15872705721558483No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAGATCGG353880.1496678151011222No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAA353310.14942674283196983No Hit
GGGTCCAGGGTTCATGTCCCTGTTCGGGCGCCAAGATCGG352040.14888961689894614No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAG350770.14835249096592243No Hit
GGCTCCATAGCTCAGGGGTAGAGCACTGGAGATCGGAAGA344030.1455019171166471No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGA327270.13841354653595644No Hit
CAGGAGATCCTGGGTTCGTATCCCAGCGGTGCCAGATCGG326640.13814709823847224No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAG325790.13778760450377134No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGG322650.13645959235440566No Hit
GGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAGATCGGA305550.12922742427983466No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACA297670.12589470589225457No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCAGATCGGA284430.12029506230703116No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAA269580.1140144952949037No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCAGATCGG267730.11323206775467234No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCG267030.112936014090801No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAA265190.11215781588862495No Hit
GGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAAGA261900.11076636366842971No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGG260150.11002622950875139No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGA259230.10963713040766335No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGG258260.1092268846162988No Hit
GGGGGATTAGCTCAAATGGTAGAGCTCTCGCAGATCGGAA257640.10896466565686991No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAG257290.10881663882493424No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAG254870.10779313901555052No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGATCGGAA251510.10637208142896815No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGGAAGATCGGAA248260.10499754656099411No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA247970.10487489575739027No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAAGATCGGA247770.10479030899628418No Hit
GGCCGTGATCGTATAGTGGTTAGTACAGATCGGAAGAGCA244310.10332695802914876No Hit
GGGTCGTGGGTTCGTGCCCCACGTTGGGCGCCAAGATCGG243020.10278137342001448No Hit
GGTAGCGTGGCCGAGCGGTCTAAGGCTCTGGAGATCGGAA238260.10076820850568942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)