FastQCFastQC Report
Sat 24 Jun 2023
SRR914334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46096938
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA33008097.160581902424842Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGATATCTCGTATG27728556.015269387307243TruSeq Adapter, Index 12 (97% over 37bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA10121932.1957922671566603No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA8315741.8039679772222614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT8108041.758910754549467Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGCACACGTCTGAACTC8083681.7536262386885653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7777391.687181478301227No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC4596110.9970532099116864Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG3106060.673810481728743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA3088620.670027150176439Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC2877740.6242800769109653Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG2557790.5548719960531869Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2491200.5404263510951638Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC2449020.5312760687054745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGCACACGTCTGAACT2318080.5028707112823849Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC1948500.42269618862753966No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1709600.37087062051713715No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA1636330.35497585544619037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT1623170.3521210020500711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1619850.35140078067658204No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1556300.3376146155304285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1367320.29661840012019886Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1332950.28916237343139795No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACAC1099330.23848221762582145No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG1026760.22273930645892356No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC986390.213981674878275No Hit
AGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGCACACGGCTGAACTC972120.21088602457716388Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACG949640.20600934491570785No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT942860.20453853138791994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA929130.20156002552707514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA893590.19385018588436395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT892950.19371134802923354Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGT888560.1927590071166983No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA839030.18201425873449556Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAG829530.17995338432240338Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG750750.1628633120924431Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG744610.16153133641978562Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG722310.15669370490508502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACGGCCCTTAAAACACCAAGGGAGGCTAAGATCGGAAGAGCACACGT707660.15351561962749025No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACA664680.1441917899188879No Hit
TGAACGGCCCTTAAAACACCAAGGGAGGCTAGATCGGAAGAGCACACGTC646020.14014379870524155No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG645250.13997675941078777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGATCGGAAGAGCACACGTCTGAAC632260.13715878481993748Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA608110.13191982513025052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGT605220.1312928854406772No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA560300.12154820348371077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAAC548770.1190469527498768Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA537460.11659342752874388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCCCTTAAAACACCAAGGGAGGCTAAGATCGGAAGAGCACACGTCTG530790.11514647675730652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG516660.11208119723700521No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC512280.11113102566595637No Hit
TTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGT503730.10927623869507341No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGTAGATCGGAAGAGCACACGTCTGA494090.10718499350217145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT482510.1046728960609054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)