FastQCFastQC Report
Tue 27 Jun 2023
SRR914335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20851539
Total Bases750.6 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT14652757.027179144906283TruSeq Adapter, Index 1 (100% over 35bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTA8165493.916013105795212No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG7519813.6063573053288778No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA6071582.9118138474095367No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA4673742.24143647142784No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT4510362.1630825427322176No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGA4465582.1416069096866184No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA3889541.8653491236306348No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG3050851.4631294121743244No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG3012631.4447998298830604No Hit
AGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGC2206041.057974665563055No Hit
TGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGA2037950.9773619107922922No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAG1987080.9529656300189642No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG1881960.902552085004373No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGA1790730.8587999187973607No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGA1740310.8346194494324854No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAG1555290.7458873898948178No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGG1545720.7412978006083868No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGA1540300.7386984720887988No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA1402530.6726266104386827No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGA1259430.6039985825506693No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA1244010.5966034449543509No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT1227980.5889157630043519No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAG1203530.5771900098117457No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGT1189350.5703895525409419No Hit
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAG1176630.5642892833953407No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGT1111140.5328815297518328No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTT1048530.5028549691224231No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAA1002350.48070792280608166No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCA969240.46482899895302693No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAG910810.43680708651769057No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG852210.40870364532804987No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA848810.40707307024196154No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAA826860.3965462693185381No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAA793630.3806097957565626No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGAT773960.3711764393026337No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGA770470.3695027019348548No Hit
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAG674480.3234677306073187No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGA662080.3175209273521729No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAG628860.3015892495992742No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGA585990.28102961608733057No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA510610.24487880726693606No Hit
TGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAG484420.23231858329497884No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCAAGATC478460.22946028108524746No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGA474130.22738369575502318No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAA460370.220784662465442No Hit
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCG458160.21972478865948455No Hit
GTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAG447710.2147131681743012No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAA441560.21176374559211195No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTT427740.20513593744807038No Hit
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATC426060.20433024152317966No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA412050.19761131300668022No Hit
GCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT410600.1969159206905543No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA407810.19557788995814648No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGA394010.1889596734322584No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTAGATCGG343810.1648847118670713No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGG338050.16212232583887454No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGA329710.1581226210688813No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGA328740.15765742758843845No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG322790.1548039211877838No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGTAGATCGGAA318170.1525882573943343No Hit
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAG307570.147504699773No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTAGTAGATCGGA284280.13633526043329464No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCG281200.13485815123766162No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTA279190.13389419361323882No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAG276750.13272401619851656No Hit
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAA272990.13092079198566592No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGG271720.13031172423292112No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCA268300.1286715575286793No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG267950.12850370421099372No Hit
CTAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGA264800.12699302435182364No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA255950.12274873331891713No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAC250870.12031246230793803No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA249960.11987604368195556No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGG241700.11591470538457617No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAG241280.11571328140335349No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGAT238830.11453830817955452No Hit
ATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGTA238600.11442800457078972No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTG235050.11272549234855038No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGA234200.1123178485770283No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG226380.10856752587902505No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAG224970.10789131679920605No Hit
TAGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAG222930.10691297174755302No Hit
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGC220280.10564208234221943No Hit
TGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAG217730.10441915102765316No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAA216610.10388202041105934No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)