FastQCFastQC Report
Tue 27 Jun 2023
SRR914336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50278819
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATG609476512.121933492511031TruSeq Adapter, Index 7 (97% over 35bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCAC24559544.884669228209199No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA21264164.229248105449733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA14919152.9672833007473787Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA9643461.9179965225515738No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC9534551.8963353136834817No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA9332941.8562369175775588No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCACA8101521.6113186747683952No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA7619441.515437345495327No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6450031.2828523279355468No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC5515161.096915184105657No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT5239311.0420511269367723Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG5144641.0232221246087743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGCACACGTCTGAACTC4895160.97360282070269Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC4792260.9531369461959717Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG4550770.9051067806505161Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA4350430.8652609759986605Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA4140110.8234302400778347Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC3252340.6468608580483961No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA3167180.6299233082622724No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCAC3161270.62874786299177No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA2591760.5154775015697962No Hit
TGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC2271390.4517588211449437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC2146630.4269451913737274No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC2038340.4054072948690382Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC1917400.38135342836911107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA1901480.3781870851023768Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTAGATCGGAAGAGCA1870220.3719697552959627No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1601530.31852975703347364Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC1501390.2986128214348074No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACA1296260.2578143293302096No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA1284010.2553779156984574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGCAC1204000.23946465409221324No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG1170160.23273418574131585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1157120.23014064829167924No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1120610.22287914121451421No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG962350.19140266600136333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT932790.18552345073976378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG931980.18536234910370508No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA907390.18047162165841646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCA893090.1776274816637996No Hit
GCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA890200.17705268693761483No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCAC888910.17679611766537318No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGA876910.17440942676079962No Hit
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATCGGAAGAGCACACGT870840.17320215894490282No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATGC841850.16743631150127053TruSeq Adapter, Index 7 (97% over 35bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAGATCGGAAGAGCACA836620.1663961120486939No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA795090.15813617260978227No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTAGATCGGAAGAGC704790.14017632355286627No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT696610.13854939591958196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC641910.12767006321290084No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAGATCGGAAGAGCACACG641430.1275745955767179No Hit
TGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT639510.12719272503198614Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT626950.12469465521853249Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACAC619890.12329048540300838No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT618140.12294242631275808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA613060.1219320604964886Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCACA604690.12026734359054854No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA601890.11971044904614805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCACA600390.11941211268307633No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAAGATCGGAAGAGCACAC584440.11623980268908067No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG577530.11486546650986372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAGATCGGAAGAGCACA576870.11473419851011218No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC532540.10591736452680003No Hit
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC532210.10585173052692427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA525590.10453507271123452Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA517640.10295388998695454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)