FastQCFastQC Report
Tue 27 Jun 2023
SRR914346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32278756
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATG538689916.688682178458176TruSeq Adapter, Index 6 (97% over 38bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA18382185.694822935555509Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA11444493.545517677323128Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG10543063.2662535074152177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG8192752.5381244556016966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT6030651.8683031031307404Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA4167141.2909853155431392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC3936321.2194769835615722Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA3626971.1236399568806184Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG3404531.0547277596447646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC3224210.998864392419584Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA3181670.9856854458703428Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT3006190.9313215168515169Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA2795970.8661950912854263Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC2409910.7465932082388801Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC2059010.6378839382781667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA2029100.6286177819244335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGAGTCGGTGTCTTGTTATCGGTAGATCGGAAGAGCACACGTCTGAACT1942850.6018974213256546Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1618310.5013545131664925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC1379100.4272469484263892No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA1256510.389268409228658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC1182080.3662098997867204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG1137530.3524082526600468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATGC940570.29138979209731625TruSeq Adapter, Index 6 (97% over 36bp)
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA934060.28937298574951276Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG925220.28663434241393937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA914700.2833752329240941Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG905630.2805653352935906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAAGATCGGAAGAGCACACGTCTGAACTC815800.2527358861041609Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA794240.2460565704576719Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG758700.23504623288456344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTCGGTGTCTTGTTATCGGTAGATCGGAAGAGCACACGTCTGAACTC695960.21560930043276763Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC694550.215172480624718No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA687960.21313089017433012Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT685100.21224485850693872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC679720.21057812760813954No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATGC672210.20825151997803137TruSeq Adapter, Index 6 (97% over 38bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT662510.2052464475396759Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGATCGGAAGAGCACACGTCTGAAC568720.17619018527231967Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA550030.17039999930604513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG545310.16893773725356703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACTC536870.1663230144309155Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAACT510840.15825888705252458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAAC507940.157360463333841Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG494050.15305732352262894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC444250.1376292196638557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA441900.13690118665043968No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT427200.13234710780056083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT410760.12725397471947184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT407500.12624402253915856Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGGCTGAA389490.12066450144485122Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT376550.11665567285182861Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATG367630.11389224541367084TruSeq Adapter, Index 6 (97% over 36bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT361040.11185065496328297Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGGATTGGCTCCAGTGGTTGGAGATCGGAAGAGCACACGTCTGAACT360490.11168026425801539Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAACT357590.11078184053933181Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTAAATCTCGTATG348720.10803390316528928TruSeq Adapter, Index 8 (97% over 37bp)
TCCAGTCGGTACGTCTGGTTCAGGGTTGTTTAGATCGGAAGAGCACACGT337660.10460750098299947No Hit
CTAAATCACTTGCATACGACTTGGTCTAGATCGGAAGAGCACACGTCTGA332540.10302131841759948Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)