FastQCFastQC Report
Tue 27 Jun 2023
SRR914347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22138948
Total Bases797 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG506722522.88828267720761TruSeq Adapter, Index 2 (100% over 35bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG9824944.437853144602896No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA9714844.388121784287131No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTA4972482.246032647983093No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA4111971.8573466092426796No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGA3847131.7377203288972898No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGA3779861.7073349646062677No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAG3169351.4315720873457944No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA3127811.4128087748342875No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG2193020.9905710063549542No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA1746300.7887908675696785No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT1686580.7618157827553504No Hit
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAG1671030.7547919621112982No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAG1656500.748228867966084No Hit
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAA1524930.6887996665424211No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGA1496620.6760122477364326No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGA1243080.5614900942899365No Hit
TTTTTTTCGAAGCATTAAGCGGAAGATCGGAAGAGC1193760.5392126129931739No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAG1169350.5281867955062725No Hit
AGAGTCGGTGTCTTGTTATCGGTAGATCGGAAGAGC1160880.5243609587953322No Hit
CCTGCAGCTGGATCATCGAGATCGGAAGAGCACACG1120900.5063022868114601No Hit
TTTTTTTCGAAGCATTAAGCGGAGATCGGAAGAGCA1078020.48693370615442066No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGA1038910.4692680067724988No Hit
TAGAGTCGGTGTCTTGTTATCGGTAGATCGGAAGAG1033330.4667475618082666No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT900430.40671760916553035TruSeq Adapter, Index 22 (100% over 36bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAA896470.4049289062876881No Hit
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACAC834090.37675231903521345No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA778670.3517195125983403No Hit
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGA688720.3110897590978578No Hit
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGC640260.28920073347658615No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGG597310.26980053433433243No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGT588090.26563592813895226No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGA571430.258110728657929No Hit
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGA571370.2580836270991738No Hit
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGA568740.25689567544040487No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCA566640.25594712088397337No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAA497290.22462223588943794No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGT463970.20957183692739148No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT461420.2084200206802961No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGA450860.20365014633938341No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT449850.20319393676700445TruSeq Adapter, Index 22 (97% over 36bp)
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAG446470.20166721562379566No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTT418930.18922760015516546No Hit
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAG417060.18838293490729552No Hit
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATC410090.18523463716523478No Hit
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAG404130.182542548995553No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA381340.17224847359504164No Hit
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCG355480.16056770177155663No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA349880.15803822295440598No Hit
TTTTTCGAAGCATTAAGCGGAAGATCGGAAGAGCAC349600.15791174901354843No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGG334290.1509963346045169No Hit
TTGTGGCTTGTCGGATGACATTGAGAAGATCGGAAG313330.1415288567460387No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATCGGAAGAG307400.13885031935573452No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGAT284230.12838460074977365No Hit
TAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAG280650.12676754107738092No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAGAT279620.12630229765208356No Hit
TATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGC276300.12480267806762996No Hit
AAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAG269710.12182602353101872No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAG249920.11288702606826666No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCGTGATAT248500.11224562251106059TruSeq Adapter, Index 12 (96% over 32bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA232840.10517211567595715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)