FastQCFastQC Report
Tue 27 Jun 2023
SRR914348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46448757
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCAC34706957.472094463152157No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG19720194.245579704102739Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA17751143.8216609327134416Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA14065063.0280810313180178Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG11069102.3830777645998147Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT10252272.2072216055211125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA9444782.033376264514463Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA8853061.9059842656284645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCACA8729311.8793420026288323No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA8237861.7735372337304958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG8129451.7501975348877474TruSeq Adapter, Index 9 (97% over 49bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC7694051.6564598273318705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC6650981.4318962292144868Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA6273351.3505958835453875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG5604371.2065705009070533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA5340541.1497702726469086Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC3835990.8258541773249174No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA3290590.7084344582138118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA3280200.7061975845769134Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC2952030.6355455324671013No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT2772780.5969546181827858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA2713410.5841727906733866No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG2483090.5345869642970209Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTG2332470.502159831747489Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC2038030.43876954554456643No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA1926530.41476459746813027No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCA1805400.38868639692554097No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACA1751340.37704776470121687No Hit
AACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC1687710.3633487974715879No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1432320.3083656253707715No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1388820.29900046625574933Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCAC1270610.2735509154744442No Hit
AAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1247480.2685712343174221No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT1220460.26275407111540144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA1219270.26249787480857667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTCAGATCGGAAGAGCA1129230.24311307189555145No Hit
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA1113090.23963827492735706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAAGATCGGAAGAGCACAC1110580.23909789448186958No Hit
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC1100430.23691269068836437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC1026640.22102636675508883No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGC1010560.21756448724774272No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGCAC998920.21505849984317124No Hit
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATCGGAAGAGCACACGT958970.20645762382834054No Hit
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC957920.2062315682634952No Hit
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA945960.20365668773439943Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT930430.20031321828482945Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAGATCGGAAGAGCACACG850680.18314375990728882No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCACA842250.18132885665810175No Hit
TTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAAC830800.1788637745462166Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG762080.16406897605462295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT744900.16037027643172455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA726850.15648427362652567No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCAC717790.1545337370384314No Hit
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCA713360.15357999784579812No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGATCGGAAGAGCACAC681820.1467897192598717No Hit
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGC641640.13813932631178916No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC627830.13516615740653729Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCACA621210.1337409308929408No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC618580.1331747155257567No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTC612720.13191311018290544No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT577750.12438438341848415Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT542450.1167846106193972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG534190.11500630684261368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCAGATCGGAAGAGCACAC497280.10705991551076383No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCAGATCGGAAGAGCACA488460.10516104876606278No Hit
ATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCAC487900.10504048579814526No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC472860.10180250894550312Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)