FastQCFastQC Report
Tue 27 Jun 2023
SRR914349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14966563
Total Bases538.7 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG500892433.467430030528725TruSeq Adapter, Index 4 (100% over 35bp)
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGAT7190604.804443077545593No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAGA4040452.6996512158469517No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAG2281301.5242644553729539No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGA1990871.3302118863228651No Hit
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGAT1358980.908010743682434No Hit
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAGA1303200.8707409977828576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG728970.4870657344642187TruSeq Adapter, Index 10 (97% over 36bp)
TAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATC661630.4420721043301658No Hit
GCGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAG470860.31460796977903344No Hit
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCG465620.31110683194264444No Hit
GGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGAT455130.30409787470910987No Hit
CCGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAG439540.29368132148977694No Hit
GCGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGA434770.29049421700894185No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTA428760.28647859899430483No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG422830.28251643346571953No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGG388320.25945836729514987No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG387090.25863653532210434No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA383770.2564182571509571No Hit
CCGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGA374460.2501977240866858No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGG370020.24723111111081414No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA354260.2367009713586212No Hit
TAACTGTAGTCGTGACTTCTAATTTGCTGGTTAGAT340130.2272599260097325No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG338700.22630446282155764TruSeq Adapter, Index 4 (97% over 35bp)
TCAACTGTAGTCGTGACTTCTAATTTGCTGGTAGAT316740.21163175540035478No Hit
AAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATC315350.21070301845520578No Hit
ACACGACGCTCTTCCGATCTAGATCGGAAGAGCACA291770.19494789819145517No Hit
GTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATC289320.19331091580612061No Hit
AGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGAT269450.1800346545830195No Hit
TGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGAT262680.1755112379508909No Hit
AACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCG262130.1751437521092852No Hit
ACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGA249300.1665713096587373No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGA248150.1658029301717435No Hit
GGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATC247540.16539535496559898No Hit
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAA239430.15997660919210374No Hit
GGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAGA236290.15787859911457294No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCG233280.15586744932687618No Hit
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAG228500.15267366328528467No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCCAAGAT217750.145490985472082No Hit
CCTGCAGCTGGATCATCGAGATCGGAAGAGCACACG215190.14378050591842628No Hit
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGA207570.138689156621998No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGA202350.13520138190712191No Hit
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACAC190320.12716346431709136No Hit
TCTGTTGAGACTATCCTTTGATTGAGTTTTTTGTAG189750.12678261535397273No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGA180580.12065562414029192No Hit
CTGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGA178250.11909882048403497No Hit
TCAACTGTAGTCGTGACTTCTAATTTGCTGGTTAGA168990.11291169522354598No Hit
CTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAG164130.10966445669590272No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGA162310.10844841263822562No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGA160910.10751299413232017No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT160170.10701855863634155No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGAT155470.10387822508080179No Hit
AAACTGTAGTCGTGACTTCTAATTTGCTGGTTAGAT154980.10355082860373487No Hit
AGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAGA150630.10064434967467147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)