FastQCFastQC Report
Tue 27 Jun 2023
SRR914350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29502767
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG13450914.559202870700229Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG10272553.4818937491524102Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA10199783.457228266080941Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCAC8022112.7191042792698057No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG7177152.432704023998834TruSeq Adapter, Index 12 (97% over 37bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA5830391.976218027278594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA5219531.7691662615916668Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT4464761.5133360203129422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA3431491.163107853578615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA3400261.1525224057797698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA3371701.142841957840768No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC3072571.0414514679250255Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG3039611.0302796344492027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA2968701.0062446007182988Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA2096510.7106147026819551Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG2029930.6880473278997865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCACA1744430.5912767436356053No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC1654200.5606931715930238No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT1578430.5350108347464494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCA1264850.42872249914728333No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA1250300.42379075833802304Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1247560.4228620318900936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1222800.4144695987328918No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC1204830.4083786446200114No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1162580.39405795395394605Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC1126400.381794697426177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA1079300.3658300931570249No Hit
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC1070690.3629117228224729Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGC923610.31305877174164715No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA906810.30736439060105786No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG900390.30518832352233266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC806640.27341164305029425No Hit
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA755330.25602005398341116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCAC754960.2558946420178148No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT701150.23765567480501068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT690330.23398822218946447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA593810.20127264673174555No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA586490.1987915235204888No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC582810.19754418288969303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACA562180.19055161842955273No Hit
TAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT561350.19027028888510694No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT491790.16669283935300036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA486590.16493029280948462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGAGTCGGTGTCTTGTTATCGGTAGATCGGAAGAGCACACGTCTGAACT479640.16257458156382418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG444550.15068078190767667No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC436910.148091194293742Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG428980.14540331081488053Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA425780.1443186667881016No Hit
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC399000.13524155208899558No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG351510.11914475682907978Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT349570.11848719138784508Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA349240.11837533747258351Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG342250.11600606817658832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCACA323330.109593110368258No Hit
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA322590.10934228643706538Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA322540.10932533887414696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC314900.1067357512602123Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTCAGATCGGAAGAGCA303780.10296661326715559No Hit
GCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA300310.10179045240061721No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT298520.10118372964813774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATCGGAAGAGCACACGT297320.10077698813809567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)