FastQCFastQC Report
Tue 27 Jun 2023
SRR914351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40297575
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG24445886.06634022022417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG14108663.5011188638522297TruSeq Adapter, Index 3 (97% over 36bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA11984362.9739655549992774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG11548712.8658573127539313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA9407282.3344531277626506Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA8623562.139969960971597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA8468472.1014837741476997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA8446932.096138539353795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA7821041.9408215010456582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC6740391.6726539996513436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA6523381.618802123949146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC6297281.562694529385453Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC6257751.5528850061076875No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT6091291.511577309552746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA5160021.2804790362695524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG4164201.0333624293769539Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA4050611.0051746289944246Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT3809250.9452802060669905Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2692860.6682436846385917No Hit
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA2034990.5049906849233484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1915500.4753387765889138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG1642480.40758780149922175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC1596580.3961975379411789Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1566120.3886387704471051Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1514060.375719878925717No Hit
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA1401730.34784475244478114Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC1255550.3115696167821513Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC1194270.2963627463935485No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTG1190030.2953105738992979Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1038770.25777481647468864Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCCAACGTAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCACA981720.24361763704143488No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT969620.24061497497057824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA959970.23822028993059755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC954270.23680581275672294No Hit
TTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT953800.23668918042835085Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG932100.2313042410120212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATCGGAAGAGCACACGT825360.20481629477704302No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC754770.1872991116711117Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT640820.15902197588812728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA640270.15888549124854287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAACT618570.15350055183221323Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAAC601540.14927449108290014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGAAACCATTTTTATCGCATTGGTTTGAGATCGGAAGAGCACACGTCT558810.13867087535664366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA550170.13652682574571795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAATGAGTTGAATACAAATGATTCTTCAGATCGGAAGAGCACACGTCTGA529050.1312858155856773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCAC520940.12927328753653292No Hit
TAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA513080.12732279795992688No Hit
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC493120.12236964631246422No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT487640.12100976299442336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT474950.1178606901283762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGTCGGTGTCTTGTTATCGGTAGATCGGAAGAGCACACGTCTGAACTC437200.10849288077508386Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCACA431300.10702877282317858No Hit
AAACCCCAACGTAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC407420.10110285792631442No Hit
TAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT405340.10058669783479528No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)