FastQCFastQC Report
Tue 27 Jun 2023
SRR914358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30171907
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGATATCTCGTATG18723016.205444687337794TruSeq Adapter, Index 12 (97% over 37bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA14441934.786548626177325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG13971274.630555834604687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA11175963.704094673233614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCAC6670992.210993822829959No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC5573801.8473476005344973No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG5341381.770315678090881Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA4938131.6366648617868271Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCAC4677231.5501936950819848No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA4203291.393113799535442Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT3866311.2814271235822117Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC3853951.277330597631764Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA3691181.223383062926715Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA3020931.0012393316736659Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA2394950.7937681897269536No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT2209800.7324031590048319Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA2002390.6636604043622433Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1962380.6503997244854294No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC1893740.6276500852266315Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG1870710.6200171570196077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA1523510.504943224172075No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCA1444850.4788726148466519No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC1438930.4769105247474082Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC1378480.4568753310819896Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1340430.4442642621164118Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA1235100.40935430432024067Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG993710.3293494176553043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG968540.321007220392135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC843840.27967738333543185No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCAC662440.2195552306322567No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA662270.21949888682873112Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA646370.2142290840283977Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC599110.19856550664828712No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG588500.19504899044001428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA559430.18541420003714051No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCACAC551610.18282238507496396No Hit
CTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA542460.1797897627087343Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACT525250.17408578118711554Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG524150.17372120363489124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT522320.1731146791616453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGAGATCGGAAGAGCACA512920.16999919826081925No Hit
TAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA509660.1689187229696817No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGAGATCGGAAGAGCACA499850.1656673540721175No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC444870.1474451051436689Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA444250.14723961597786975Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTCTTTACTTATTCGGTTGAAAGAGAAGATCGGAAGAGCACACGTC443780.14708384193282845No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA429230.14226147521931579Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC426430.14133345963183566No Hit
AGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACTC419990.13919902378063145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC419460.139023363687287No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA417290.13830415160698992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG410730.13612994365917938No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT381850.1265581257425989Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAATCACTTGCATACGACTTGGTCTAGATCGGAAGAGCACACGTCTGA375940.12459934998473911Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGCAC359590.1191804018221321No Hit
TAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT355320.11776517805122493Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGT333700.110599571979325Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGC331440.1098505308265732No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT329120.10908160362551828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG325550.10789838375148114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCATCGACTCTTTACTTATTCGGTTGAAAGAGAAGATCGGAAGAGCACAC323790.10731505966792222No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT318280.10548885756541673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG316950.10504805016136368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAGATCGGAAGAGCACACG309670.10263520963391543No Hit
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT309080.10243966349226781Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC304040.10076923543480365Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)