FastQCFastQC Report
Tue 27 Jun 2023
SRR914359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19031000
Total Bases685.1 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA273885014.391519100415112TruSeq Adapter, Index 7 (100% over 35bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA9694385.093994009773527No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA6619243.4781356733750197No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG6343823.333413903630918No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGA6110663.210898008512427No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA4229772.2225684409647415No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGA3826022.010414586726919No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGA2962951.5569071514896746No Hit
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAG2661071.398281750827597No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTA2616891.3750669959539699No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG2119781.1138563396563501No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAG1903891.0004151121853817No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA1866850.9809521307340654No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGA1841810.9677946508328517No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAA1760560.9251011507540329No Hit
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAA1487790.7817718459355787No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGG1420970.7466607114707582No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA1266160.6653144868898114No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAG1180490.6202984604067049No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGG1174370.6170826546161525No Hit
CCTGCAGCTGGATCATCGAGATCGGAAGAGCACACG973250.5114024486364354No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG910310.4783300930061478No Hit
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAG885620.46535652356681206No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTT824230.43309862855341286No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAA813160.4272818033734433No Hit
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATC783980.4119489254374442No Hit
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACAC730300.3837423151699858No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAG726510.38175082759707846No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGA700590.3681309442488571No Hit
TTTTTTTCGAAGCATTAAGCGGAAGATCGGAAGAGC669940.35202564237297046No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGA660630.3471336240870159No Hit
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGA543330.2854973464347643No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGA507920.26689086227733694No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGAG491550.2582891072460722No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA451750.23737586043823233No Hit
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCG444560.23359781409279598No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA444440.23353475907729493No Hit
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGA441800.23214754873627239No Hit
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGG439310.23083915716462616No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGAT434210.2281593190058326No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGA428520.22516946035415902No Hit
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGC423970.22277862434974516No Hit
AGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGA413570.21731385633965636No Hit
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAG413350.21719825547790444No Hit
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAG390520.20520203877883453No Hit
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAA374200.19662655667069517No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC368510.1936366980190216TruSeq Adapter, Index 7 (97% over 35bp)
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGG342070.17974357627029583No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGA330110.17345909305869373No Hit
TCCAGTCGGTACGTCTGGTTCAGGGTTGTTTAGATC290160.15246702748147758No Hit
CTAAATCACTTGCATACGACTTGGTCTAGATCGGAA286520.1505543586779465No Hit
AGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGC278560.14637170931637855No Hit
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGA256800.13493773317219274No Hit
TAGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAG255820.13442278387893436No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAAGA250230.13148547107351163No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTT243670.1280384635594556No Hit
AATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGC231820.12181178077872944No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG231590.12169092533235248No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAG227970.11978876569807156No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTA227230.11939992643581523No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAGAT225120.11829120907992223No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGAG221630.11645735904576744No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA220860.11605275602963586No Hit
TCGACTCTTTACTTATTCGGTTGAAAGAGAAGATCG220770.11600546476801009No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA216170.11358835584047082No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGA213500.11218538174557302No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAG211200.11097682728180337No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGAT204830.10762965687562398No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA201560.10591140770322106No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGA198850.10448741526982291No Hit
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAG197130.10358362671430825No Hit
CTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAG196150.10306867742104987No Hit
ACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGA194890.10240659975828911No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGG191670.10071462350901161No Hit
TAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAG191250.10049393095475803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)