FastQCFastQC Report
Tue 27 Jun 2023
SRR914360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34005616
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG31956649.397459525508962TruSeq Adapter, Index 9 (97% over 49bp)
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA10806513.1778603863550066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG10315143.033363665578062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG10009512.9434873345626205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA9510702.7968027398768487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA8886482.6132389426499434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA7785662.289521824865634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT7324732.15397656669416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA7081612.0824824934799Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC5956421.7515989123678866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG5532821.627031252720139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC3557781.0462330692671469Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA3427841.0080217338218487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA3198950.9407122635272951Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA2325760.6839340890045927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA2260040.6646078694766182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATCGGAAGAGCACACGT2136380.6282432878145774No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1777710.5227695331265283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT1697000.4990352181827849Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT1080480.3177357528238865Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGACCTCCAGTCACGATCTGATCTCGTATG1077710.3169211814895516TruSeq Adapter, Index 9 (97% over 37bp)
AACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC1061240.31207786384460734No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC979400.28801125084750706Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA958120.28175346095774295No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT929520.2733430854480036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC857600.2521936376626731Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA850240.2500292892797472Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG812010.2387870285896306TruSeq Adapter, Index 9 (97% over 37bp)
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC797540.23453184909222052Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC790570.2324821876480638No Hit
AGATCNGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG715650.21045053264143193TruSeq Adapter, Index 9 (97% over 37bp)
TTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAAC700010.20585129232771432Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT688170.20236951449431176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC661220.19444435295628815Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTC590540.17365955082242887No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT581900.171118794025081Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT564920.1661255011525155Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA541800.15932662416701993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG525090.15441272994437155No Hit
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC512100.1505927726761368Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCTGCGGACGGCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC486300.14300579057294538Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG482140.14178246322607418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACTC464730.1366627206517888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG453830.13345736774772732Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAAC449040.13204877688438285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGAAGATCGGAAGAGCACAC433350.12743483311697693No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTG424710.1248940763196291Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTCTTTACTTATTCGGTTGAAAGAGAAGATCGGAAGAGCACACGTC414910.12201219939671142No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT376340.110669955221514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACTC355400.10451214881683071Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGAGATCGGAAGAGCACACGT345080.10147735597555414No Hit
TCCAGTCGGTACGTCTGGTTCAGGGTTGTTTAGATCGGAAGAGCACACGT343460.10100096407605144No Hit
TTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAACT340580.10015404514360216Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)