FastQCFastQC Report
Tue 27 Jun 2023
SRR914361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20407402
Total Bases734.6 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGA17415708.534011335690844No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC15677257.682139059151185TruSeq Adapter, Index 5 (100% over 35bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA7337923.5957149273582205No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG6452583.161882144527755No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA6164813.02086958447724No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAA5794592.839455017351057No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA4860942.3819494514784387No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGG4317012.1154138091659096No Hit
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAA3673401.8000331448363687No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG2395761.1739661912868673No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA2120281.0389759558811063No Hit
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGA2043301.0012543487897185No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGA2023000.9913069777328833No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGA1837350.9003350842993146No Hit
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAG1836760.9000459735149041No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAG1754370.8596733675359559No Hit
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGA1395370.6837568054963586No Hit
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAG1360240.6665424633669685No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA1326370.6499455442686923No Hit
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAG1217470.596582553722419No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAG886440.4343718029369932No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA801870.3929309571105622No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAA781240.3828218800217686No Hit
AGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGA769560.3770984665269984No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGA743670.3644118932924436No Hit
TTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAA712970.34936833213752544No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGG689720.33797540715863783No Hit
CTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAG661750.3242695959044664No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG641750.3144692303312298No Hit
TAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATC491330.24076068085491725No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA472120.23134742972182346No Hit
TAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAG423890.2077138481419634No Hit
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAG417960.20480803974949874No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA380150.18628044863329493No Hit
TAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAG378610.1855258204841557No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGA357350.1751080318798052No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA348620.17083017230708739No Hit
AATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGC347840.17044795804973117No Hit
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGC335280.16429332846973857No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGG332870.16311238441816356No Hit
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAG311440.15261129270644053No Hit
AGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAG310710.1522535793630174No Hit
CTAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGA299170.14659876842725986No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA284280.1393023962579852No Hit
TAGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAG277760.13610747708111007No Hit
TGCGTGGGTTCGAATCCCACTTCGTGCACCAAGATC259970.1273900519037161No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACA257230.1260474018201827TruSeq Adapter, Index 5 (100% over 36bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA247410.12123542232372353No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGG242730.11894213677958615No Hit
CTGTAGTCGTGACTTCTAATTTGCTAGATCGGAAGA241730.11845211850092432No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGG235520.11540910499043436No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAA227620.11153796058900589No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGAT218340.1069905909630241No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATCGGAAGAG211700.10373686959270956No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)