FastQCFastQC Report
Tue 27 Jun 2023
SRR914362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59376273
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG28245304.757001167789699Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG27939484.7054957457501585TruSeq Adapter, Index 12 (97% over 37bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG18023923.035542496916235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT15275312.5726286323158076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA8569841.4433105291064665Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG7491271.2616605289456277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG6880231.1587507353316029Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA6432421.0833317207363284Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA6295601.0602888463545026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA5360770.9028471692724802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT5304910.8934393709756757No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA5293440.8915076229186698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG5025030.8463026973754314Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA4697220.7910937757915524Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT4159650.7005576116237542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC2604910.4387122782192813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACG2499630.4209812899506171No Hit
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2435060.4101065757360688No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA2402270.4045841678207051Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT2242070.37760369365049234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA2011180.3387177905221501Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC1783400.3003556656376866No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1759770.29637596148885936Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1697440.28587850234385714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA1675530.28218847619485987Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC1621810.2731410912234252No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTC1503080.25314488836306714No Hit
ACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAG1499330.2525133229564611Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT1420620.23925718611540336No Hit
TTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA1418810.23895235054581482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT1313130.22115399530044602Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG1231170.20735050177366302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA1220190.20550127826312034Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC1173060.19756376423289485No Hit
AACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC1107200.18647179151847407No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCAC1004040.16909784822634458No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC990350.16679221344862788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT981120.16523772046116805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA977200.16457752408946247No Hit
AGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC952370.16039571901052127No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC876410.14760273013430802No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGATCGGAAGAGCACAC857940.14449205998497075No Hit
TGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACG850870.14330134867171607No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA838280.14118097307993718Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG826750.1392391199764256No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT799940.13472384836279636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGT774040.1303618366211702No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC757000.12749200341355207Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC729010.12277799921864412No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCAC727370.12250179461415504No Hit
TAGTTGGATCTAGGTTACGTGCTGCAGTAGATCGGAAGAGCACACGTCTG724220.12197127967260592Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG712960.12007489927837Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACAC694030.11688675710582239No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG684180.11522784530447036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGA665000.11199759877148234Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT656710.11060141817927843Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTCTTTACTTATTCGGTTGAAAGAGAAGATCGGAAGAGCACACGTC642900.10827557330855037No Hit
TCATCGACTCTTTACTTATTCGGTTGAAAGAGAAGATCGGAAGAGCACAC642800.10825873156437421No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT625760.10538889835675608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACTCTTTACTTATTCGGTTGAAAGAGAAGATCGGAAGAGCACACGT622230.10479438478733752No Hit
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT620390.10448449669449612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC615150.10360198929966521No Hit
TCGAGAAGAGTGGGGACTGTGGCTCCGAGGTTAGATCGGAAGAGCACACG612590.10317084064875544No Hit
GTGACTTCTAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACG600010.10105214923139416No Hit
TCGTAGTTGGATCTAGGTTACGTGCTGCAGTAGATCGGAAGAGCACACGT598410.10078268132457556No Hit
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA598380.10077762880132271Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)