FastQCFastQC Report
Tue 27 Jun 2023
SRR914363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46699245
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG26732315.724355929094785Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA18437933.948228713333588Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG18228153.9033072162087414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA14676283.142723185353425Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT10183072.180564161155068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG9552072.0454442036482603TruSeq Adapter, Index 8 (97% over 37bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA9028311.9332882148308823Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA8898731.9055404428915286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA8398821.798491603022704Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA6317751.3528591308060762Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC5698961.220353776597459Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCAC5598181.1987731279167362No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA4797371.027290698168675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG4420730.946638430664136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA4395100.941150119236403No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG4270360.9144387666224583Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT3701930.7927173126674746Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC3423980.7331981491349593No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC3355220.7184741423549781Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCAC3146270.6737303782962658No Hit
TTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG2868460.6142411938351465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA2806710.6010182819872142Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC2490810.5333726487441072Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA2290860.4905561107037169Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA2238380.4793182416546563Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA2106250.4510244223434447No Hit
AGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1993650.4269126834919922No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC1665160.35657107518547676Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1412590.30248668902462983No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1342950.28757424236730167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC1336860.2862701527615703Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA1242940.26615847857925756Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC1210230.2591540826837779No Hit
CTGTAGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT1200630.25709837493090093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCA1122420.24035078083168154No Hit
GTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA1021900.21882580756926584Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT1009770.2162283351690161No Hit
TTGTAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA1003130.21480647063994288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTAGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC939210.20111888318537055No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG927610.19863490298397757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC863810.1849730118763162No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCGGAGATCGGAAGAGCACAC851870.1824162253586755No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCGAGATCGGAAGAGCACA812700.17402850945448906No Hit
ACTGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGA700750.1500559591488042Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT642720.13762963405511158Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA625110.13385869514592796No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG622500.13329979960061453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAACT578480.12387352300877669Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT541150.11587981775722499Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAAC537150.11502327286019291Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAGTCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAAC517740.11086688874734484Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCCCAACGTAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA490910.1051216138505023No Hit
GCGGAAACCATTTTTATCGCATTGGTTTGAGATCGGAAGAGCACACGTCT478870.10254341371043578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGTCGTGACTTCTAATTTGCTAGATCGGAAGAGCACACGTCTGAAC473890.10147701531363086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA472430.10116437642621416Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)