FastQCFastQC Report
Tue 27 Jun 2023
SRR914370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31461064
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG9623813.0589588451299674Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG8110372.5779070917626945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT7010412.228281281268809Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA5467071.7377257170958997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA5048011.6045261533430655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA4592521.4597471973611573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATG4483451.4250789483788595TruSeq Adapter, Index 6 (97% over 38bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG3750331.192054407314387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA3543061.1261729736794661Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA3276061.0413061681575677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT2513700.7989875994022325Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2306370.7330870945750595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC2126370.6758735178187235Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA2111640.6711915401208299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA2095050.665918355463121Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1803760.5733308956111592No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1800050.5721516602235703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG1601260.5089656217602813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA1269950.40365767667616076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACG1184530.37650665597323724No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1176370.37391297382695005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG1145500.3641008454132384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC1059930.33690214672968466Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACG974290.30968119832183677No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA947700.30122948162210916No Hit
TGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA858540.27288968993547075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC846150.2689514887354096Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGA805830.2561356475419903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGAA776230.24672719269761503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGT767990.2441080822949917No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC735230.23369521132533852No Hit
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGT687390.2184891140363212No Hit
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC668980.21263743654696485No Hit
TTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA651150.20697011391604556Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGAA625210.19872500179904914Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC604960.19228847441396132No Hit
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACAC597880.1900380737282121No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT574790.182698843243191Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC563800.1792056365290125No Hit
TTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTG541980.17227007961332774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCT530910.1687514446428131Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG510920.16239755909081777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC506920.1611261462740103No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC488910.15540161006633468No Hit
TTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA484760.15408251926889693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAAC470030.14940054157100344Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACG460560.14639047172721176No Hit
ACTGTAGCCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTC454480.14445792424566442No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA450800.14328822445420156No Hit
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT448540.14256987621270534Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACTC444950.14142878320962063Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC413080.13129880159170715No Hit
TAGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACT412380.13107630434876583Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC382930.12171552748502085Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA377880.12011036880380142Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT353270.11228800144839349Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTAGCCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGA348100.11064470038266982Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT338310.10753291751353355Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGTAGCCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA331050.105225303251028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT327910.10422724418983415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)