FastQCFastQC Report
Tue 27 Jun 2023
SRR914371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51975404
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA13537952.6046839385798712Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC9283791.7861890982126853No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG8700541.673972558250822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG8583931.6515369462063247TruSeq Adapter, Index 8 (97% over 37bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7494521.4419358818259498No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC7336961.411621543143753No Hit
TTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT6816241.311435693698504Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6559101.2619622927798695No Hit
TTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC5894631.1341191306564928Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA5817981.119371770539773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC4461480.8583829382066949No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA4194780.8070702057457793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG3705450.7129237513959487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC3335090.6416669700152788No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA3014340.5799550879873873No Hit
TTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2749040.5289117137021195Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA2579160.49622702307422184Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA2254720.43380518985480127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA2191150.42157440469342Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA2179640.41935989569220083No Hit
CAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG2073270.39889444630387094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT2059970.39633554363521634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA1954510.37604517706105756Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA1877260.361182377726203No Hit
TTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA1788130.3440338818722794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1723670.33163186187066485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC1697840.326662203529962Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1421080.27341394017831977Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1292840.2487407312889766No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACA1144580.22021570048786923No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAGATCGGAAGAGCACA1102580.2121349552184337No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCACA1083580.2084793799774986No Hit
TGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA1043360.20074110438852963Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCT956400.18401011370686027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACTC897930.1727605618996247Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA893890.17198327116418374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGCAC830880.15986022927306154No Hit
TGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGAA821990.1581498048576977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAATGAGTTGAATATAAATGATTCTTCAGATCGGAAGAGCACACGTCTGA810460.15593144788254076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC788100.15162941302005079Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT780050.15008060351007566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC742390.14283486858514846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG723740.1392466328881253No Hit
TTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG698040.13430198637801835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA618130.11892740650943281No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCAC607010.11678793300000131No Hit
AATGAGTTGAATATAAATGATTCTTCAGATCGGAAGAGCACACGTCTGAA589950.11350561123103535Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACAC560450.10782984967274137No Hit
CTGTAGCCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA554290.10664467369989082Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC520300.1001050419925548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)