FastQCFastQC Report
Tue 27 Jun 2023
SRR914372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47932299
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG639081013.332992853107253TruSeq Adapter, Index 10 (97% over 36bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA21636084.513883216826299Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG12090132.5223346787517955Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC11287942.354975712723481Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT10677192.227556412430791Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA9853452.055701521848556Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC9406631.9624825423040944No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6729121.403880085117553No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG6632861.3837975933514057Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC5881301.227001442179938No Hit
TTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC5494401.1462834277988627Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA5422541.131291449216738Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA5094001.0627489409594144No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA4778020.9968267952263253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA4543090.9478139156229498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA3885920.8107101226252469Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA3754240.7832380416386872Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC3640920.7595963631955146No Hit
ACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT3350380.6989817033395374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA3139840.655057250644289Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC2974880.6206420434788659No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA2920540.6093052202649407No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA2653870.5536705009705459Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA2552480.5325177496702171No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC2162630.45118428390009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG2025720.42262108062039755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG1795610.37461378599845585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACA1634800.3410643833294956No Hit
TGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA1467010.3060587600857618Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG1448590.30221583988700396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC1268200.2645815090154553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1237890.25825800677743416No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAGATCGGAAGAGCACA1202420.25085798617754596No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCACA1199330.2502133269259628No Hit
TTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT1197670.24986700512737767Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC1182650.24673341873295082No Hit
TCAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCT1142540.23836536611774034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGAA1127300.2351858816536215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA1077290.2247524159022708No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG851690.17768603170901523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACTC787540.16430257184200575Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA784090.16358280665819933Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC772760.1612190560690611No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT755780.15767655959919635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT751550.15679406489557282Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAGATCGGAAGAGCACA727450.15176613998840324No Hit
TTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA702120.14648160314613745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGCAC692020.1443744644920954No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGATCGGAAGAGCACAC691590.1442847546286065No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACAC664590.13865180971186047No Hit
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC653900.13642158078000807No Hit
GTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA639190.13335266893832903Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAATGAGTTGAATATAAATGATTCTTCAGATCGGAAGAGCACACGTCTGA607890.12682262538669384Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATGC597780.12471340045675672TruSeq Adapter, Index 4 (97% over 35bp)
CTGTAGCCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA593560.12383299202902828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGAA563610.11758459572323038Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG562510.11735510537477037No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC557990.11641210867018917No Hit
TAGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACT549110.11455949567534826Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGGTTCGTGGCGGCTAGCGTTTAGTAGATCGGAAGAGCACACGTCTGA532100.11101074037779828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCAC531370.11085844223745663No Hit
TTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA522570.10902251944977645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATGC515600.10756838515089795TruSeq Adapter, Index 10 (97% over 36bp)
AATGAGTTGAATATAAATGATTCTTCAGATCGGAAGAGCACACGTCTGAA500460.10440976344573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGTCGGTACGTCTGGTTCAGGGTTGAATAGATCGGAAGAGCACACGT497960.10388819447195721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)