FastQCFastQC Report
Tue 27 Jun 2023
SRR914373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41937377
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG12368892.9493713924931453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATG11840442.8233620810381157TruSeq Adapter, Index 7 (97% over 35bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT9264402.209103349501329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG8996362.1451890040714754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC7942601.893919116591388No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7932511.8915131482829743No Hit
TTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA6700481.597734641343926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA5849471.3948106482672962No Hit
TTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA5316401.2676996942369572Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA4318071.0296471331528436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG3726460.8885772708197749Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC3586460.8551941624770666No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA3533960.842675496848551Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA3450740.8228316234465498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA3009490.7176152194735499Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC2917640.6957135158929945No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG2551870.6084953763321916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA2449630.5841161692110597No Hit
TGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA2441530.5821847179426601Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA2377470.5669095613681323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC2280750.5438466025187985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2122340.5060736154290241Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC2108530.5027806102417898No Hit
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACG2087250.4977063777736982No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA1990330.474595728769589No Hit
TTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT1692090.4034801699686654Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1542110.36771732290266984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC1513360.3608618631537208No Hit
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACG1481790.3533339722224401No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1403130.33457743434931564No Hit
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGT1367620.3261100473689616No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA1242180.296198782293895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTG1237040.29497314531616986Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA1216150.28999190864989005No Hit
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACG1202260.28667982740074566No Hit
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACAC1132280.2699930422448691No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC1007020.24012469830910024No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA1006680.24004362504598223No Hit
CAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG984560.23476909392783435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA944240.22515475872513438Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG916350.21850436664171916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC890940.2124453324775176No Hit
TAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC849780.20263069862476138Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCA818790.19524110914232903No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCA788180.18794213095396978No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT772400.1841793777421988Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCACA759300.18105567260441682No Hit
TGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA756910.18048577525485202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT726750.17329409991473715No Hit
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC703760.16781211662331671No Hit
GGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGT694610.16563029204234686No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC672960.1604678327879209Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC670140.1597954016055892No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG661820.15781149116693682No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACA627350.14959209299141432No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCAC624990.1490293491650658No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGCAC600730.14324453339082224No Hit
ACTGTAGCCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTC594840.14184005833268973No Hit
AACTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT573550.1367634413568593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAAC527760.12584478042105496Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC516430.12314313315303434No Hit
TCAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCT498750.11892732347089806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT486430.11598960993673972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA482260.11499527020967477Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGCCGTGACTTCTAATTTGCTGGTTTGAGGTAGATCGGAAGAGCAC478830.11417738405527843No Hit
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGT443680.1057958393535199No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTAGATCGGAAGAGCACAC436930.1041862966298536No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGTTTGAGGAGATCGGAAGAGCACA436700.10413145295186202No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT423230.10091952102774572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)