FastQCFastQC Report
Tue 27 Jun 2023
SRR914374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43271007
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG23662005.4683266326572895TruSeq Adapter, Index 10 (97% over 36bp)
CTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG15884553.6709453052479226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA14868643.4361668541709696No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG10354452.392930213063911Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA9319222.153686878606731No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA8048091.859926670992427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA7947921.83677722129277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT7484081.7295830439074364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA5740991.3267521137190081Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA4543801.0500795602006674Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC4122410.9526956467641255No Hit
ACTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG3911840.9040325777488839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT3086990.7134084029983402Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2621550.6058444630142302Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA2503100.5784704756235509Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA2342720.5414063971286824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACG2260000.5222896707719328No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC1981930.45802724212080387No Hit
TTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA1968090.4548287956413864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA1921290.4440132396271711Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG1830590.4230523223090232Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG1815400.41954188863688796No Hit
TTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG1784960.4125071551951634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC1756230.4058676055308812Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACG1568660.3625198738730532No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1539860.3558641470950745No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA1497900.3461671229421585No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1495220.34554777058920766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC1441280.3330821489779519No Hit
TTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1336440.30885345469311587Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC1328650.30705317304032237Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC1317130.3043908823291309Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC1270840.2936931881432757No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1182630.27330771386947383No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC1059700.24489839120222No Hit
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGT1013200.2341521656752754No Hit
CAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG1010980.23363912006947285Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA985160.22767207613171564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGAA980500.22659514256277882Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCT977600.22592494785249623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGT960060.22187142536340787No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA872510.20163847816160135No Hit
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACAC867440.20046679292672806No Hit
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC865470.2000115227269844No Hit
CTGTAGCCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA849180.1962468772681902Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT801630.18525799503579843No Hit
TTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA778990.1800258542631097Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGCCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT773440.1787432402486034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACT725980.16777515716239283Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGA710480.1641930819867446Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC693340.1602320001473504No Hit
TTTTTCGAAGCTTTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC684150.15810817622062737Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACG670540.15496288311478398No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC660400.15261951264503734No Hit
TGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGT643570.1487300723091561No Hit
GTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA619730.14322060958738492Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA619310.14312354690520607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCTTTTTTCGAAGCTTTAAGCGGAGATCGGAAGAGCACACGTCTGAA607500.14039423672298637Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA585230.13524760355126472No Hit
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT576540.13323933043665936Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC551610.1274779669444716No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCAC525930.12154327723410735No Hit
AGAGTCGGTGTCTTGTTGTCGGTAGATCGGAAGAGCACACGTCTGAACTC523600.12100481044963894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA510680.11801897746451799No Hit
AATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA507380.11725634210454126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG485700.11224605889111848No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC482790.11157355316459357No Hit
TTACCCCAACGCAAGGCGGGGGGCAATTATTAGAAGATCGGAAGAGCACA467170.1079637457940371No Hit
CCTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG441770.10209376453845873Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGCCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGA440990.10191350527155515Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAAC440350.1017656002320445Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT438110.10124793259375729Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)