FastQCFastQC Report
Tue 27 Jun 2023
SRR914375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR914375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23285884
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCAC8679623.727417005083423No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC8246473.541403023393915No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7708123.3102114568637377No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7503463.222321299891385No Hit
CTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG6122692.6293569099631346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC4835242.076468301568452No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC3827131.6435407820463248No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA3338721.4337956849737807Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA3139871.3484006018410124No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG3033951.302913816799912Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCACA2971201.2759661604429533No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA2523821.0838411803477161Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA2354761.0112392555077574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG1428700.6135476754930154Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA1387720.5959490307518495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1372490.5894085876233001Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA1329560.570972525672635Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG1305570.5606701467721819No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA1302350.5592873347647012Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCA1277850.5487659390556099No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG1267960.5445187307469195TruSeq Adapter, Index 12 (97% over 37bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG1252510.5378838097793496No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1238720.531961767051661Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA1057530.45415067772389484Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC1020350.4381839229294452Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACA953170.40933382645039373No Hit
ACTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT929090.39899279752488676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTTAGATCGGAAGAGCA903710.3880934904597137No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAGATCGGAAGAGCACA853880.36669425992159027No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCACA835090.3586249935798014No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGCAC781130.3354521563364311No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC715780.3073879437001404No Hit
TTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT684870.2941138073177724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG632850.2717740928366731Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA588010.2525177914654217No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC583790.250705534735121No Hit
AATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA579170.2487215001156924Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAATGAGTTGAATATAAATGATTCTTCAGATCGGAAGAGCACACGTCTGA566020.24307430201060864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAGAGATCGGAAGAGCA565720.24294546859376262No Hit
TTTTTCGAAGCTTTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC539120.23152223896674914Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAATGAGTTGAATATAAATGATTCTTCGAGATCGGAAGAGCACACGTCT493810.21206409857577233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG478780.2056095443917869No Hit
TGTAGCCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA440440.18914463371886592Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAAGATCGGAAGAGCACA433710.18625447073428691No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACAC426820.18329559659405673No Hit
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAGTAGATCGGAAGAGC415890.17860176577363351No Hit
CTTTTTTCGAAGCTTTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG404460.17369321259180023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGTTCGTGGCGGCTAGCGTTTAGTAGATCGGAAGAGCACACGTCTGA402440.17282573425170372Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG372940.16015711492851206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCAC370490.15910497535760293No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA369120.15851663608733943No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCA350990.15073080326261182No Hit
TTTTTTTCGAAGCTTTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT322650.1385603398178914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAGTTGAATATAAATGATTCTTCAGATCGGAAGAGCACACGTCTGAA306930.1318094687751601Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGA303480.1303278844814309No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTAGATCGGAAGAGCAC303400.1302935289036053No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGATCGGAAGAGCACAC301850.1296278895832342No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC298730.12828802204803563No Hit
CGGCCTTTTTTCGAAGCTTTAAGCGGAGCCAGATCGGAAGAGCACACGTC271190.11646111438157125No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC259990.11165133348598662No Hit
GTAGCCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA258720.11110593868800514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGCCGTGACTTCTAATTTGCTGAGATCGGAAGAGCACACGTCTGAA254090.10911760962134828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)