FastQCFastQC Report
Sat 30 Sept 2023
SRR9257373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9257373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13698449
Total Bases753.4 Mbp
Sequences flagged as poor quality0
Sequence length55
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG10657547.78010707635587TruSeq Adapter, Index 2 (100% over 49bp)
AGGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCT2224151.62365096953677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACAC1004900.733586700216937No Hit
TGGTTCGATCCTTCCCAGTAAAACGCGGAGATCGGAAGAGCACACGTCTG986380.7200669214449023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTC957480.6989696424755825No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG910990.6650314937114413No Hit
GATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGT895760.6539134466975057No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAATCTAGATCGGAAGAGCACACG750040.5475364400743471No Hit
AAGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGT708510.5172191391886776No Hit
AAAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACACG692510.5055389847419952No Hit
TGGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACTC588540.4296398811281482Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGT561050.4095719157694422No Hit
CCAATGATCACCCATGACTTCTGTGTACCAGATCGGAAGAGCACACGTCT542590.39609593757658257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTCC538630.39320509935102876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACTCC472430.34487846032788094Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT444660.32460609226635806No Hit
AGTTCTTCCTATTTGACCCGAAACCGATCGATAGATCGGAAGAGCACACG430870.31453925915262376No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGTC409180.2987053497808402No Hit
ACCAATGATCACCCATGACTTCTGTGTACCAGATCGGAAGAGCACACGTC407340.29736213201947165No Hit
GAGCGTAATGGTATAAGCCTGCCTGACTGTAGATCGGAAGAGCACACGTC372840.2721767989938131No Hit
AGGTGTGAAGCCATCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT365880.2670959318095063Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGATATAGCTGGTTTTCCGCGAAATCTAAGATCGGAAGAGCACAC364960.2664243229288221No Hit
AAAACGCGGCGTGTTCGAATTCTGATAGATCGGAAGAGCACACGTCTGAA363360.26525630748415385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGT355770.25971553421850896No Hit
AGCGTAATGGTATAAGCCTGCCTGACTGTAGATCGGAAGAGCACACGTCT341110.24901359270673634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGA326200.23812914878173436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTTCTTCCTATTTGACCCGAAACCGATCGATAGATCGGAAGAGCACAC306090.2234486546615606No Hit
ACCGAAGCGACGAGACCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG303030.2212148251236326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTC298100.21761587753474865No Hit
AAGATCGGATATAGCTGGTTTTCCGCGAAATCTAGATCGGAAGAGCACAC283380.20687013544380098No Hit
AAAACGCGGCGTGTTCGAATTCTGATCGCAGATCGGAAGAGCACACGTCT282210.20601602414988734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGATGATGGAAAAACCAGAGATCGGAAGAGCACACGTCTGAACTCCAG279550.2040741984731264Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTG278470.20328578804797537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACG262020.1912771292574802No Hit
AAGATCGGATATAGCTGGTTTTCCGCGAAATCAGATCGGAAGAGCACACG261000.19053251941150418No Hit
GATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTCT254640.185889658018948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACGCGGCGTGTTCGAATTCTGATCGCTAGATCGGAAGAGCACACGTC248350.18129789730209603No Hit
AATGATCACCCATGACTTCTGTGTACCAGATCGGAAGAGCACACGTCTGA244880.1787647638064718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTCAAGTGATTCACCGAAGCGACGAGACAGATCGGAAGAGCACACG231730.16916513687060483No Hit
GTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAAC231400.168924233685142Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTG224250.16370466466678088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGATATAGCTGGTTTTCCGCGAAATCAGATCGGAAGAGCACACGT218970.15985021369937574No Hit
GGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTCCA209420.15287862151401227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTC208240.15201721012356947No Hit
ACCGAAGCGACGAGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT201370.14700204380802526Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGTC200170.14612603222452408No Hit
AGGCCGTTCTCGTTTGTACAGACAGACTTAGATCGGAAGAGCACACGTCT195970.14305999168227002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATG186130.13587669669756045TruSeq Adapter, Index 2 (97% over 49bp)
GAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTGAACT185090.1351174866585261Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAAAGATCGGAAGAGCACACGTC177920.1298833174471066No Hit
GTGATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACAC177670.1297008150338772No Hit
AAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCTGAACTCC175330.1279925924460499Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACACGT169390.12365633510771915No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT164990.12044429263488152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGTGTAGCGCAGTCTGGTCAGATCGGAAGAGCACACGTCTGAACTCC164920.12039319195917729Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACA164530.1201084881945394No Hit
GGGAGAGGTGAGCCAGGATCGAACTCTCCATGAGACAGATCGGAAGAGCA152760.11151627457969876No Hit
TGGTTCGATCCTTCCCAGTAAAACGCGGCAGATCGGAAGAGCACACGTCT151020.11024605778362206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGTCTGAAC150390.10978615170228395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAAGATCGGAAGAGCACACGTCT150300.10972045083352136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCTTCCTATTTGACCCGAAACCGATCGAAGATCGGAAGAGCACACGT149180.10890284002225362No Hit
CTTGGAGCGAATTGGATGATCGGGCCGAGGGAGATCGGAAGAGCACACGT148390.10832613239644868No Hit
GAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAACTC147470.10765452351576446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTC147310.10753772197129763No Hit
TGGTCCGAAAGGGCGTGGGTTCAGATCGGAAGAGCACACGTCTGAACTCC146270.10677851193226329Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTGACCCGAAACCGATCGATAGATCGGAAGAGCACACGTCTGAACTCC142470.10400447525117625Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTGAA139810.10206264957441531Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)