FastQCFastQC Report
Sat 30 Sept 2023
SRR9257375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9257375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22849989
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length55
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACTC5586912.4450383761672705Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGT4895052.1422548606040905No Hit
AGGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCT4609052.0170906865644445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4365621.9105567184299301TruSeq Adapter, Index 9 (100% over 49bp)
AGGTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTC3679411.610245851759491No Hit
GATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGT2680581.173120914850331No Hit
GGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACTCC2620431.1467970509745102Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGT2473231.0823768886715874No Hit
TGGTTCGATCCTTCCCAGTAAAACGCGGAGATCGGAAGAGCACACGTCTG2393961.0476854058879417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATGAGTGTTCGCCCTTGGTCTAAGATCGGAAGAGCACACGTCTGAACT2276270.996179910633655Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTCT2173710.951295862768249Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTC2095010.9168538330587381No Hit
TGATCCGGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTCT2004110.8770726322887945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGTC1910560.8361316935426096No Hit
AGGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTCC1829160.8005080440082487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTGTGAAGCCATCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1824730.7985693122215507Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACACG1731220.7576458789542525No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG1679660.7350813166693428No Hit
AGGTGTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGT1582260.6924554755803165No Hit
AAGATCGGATATAGCTGGTTTTCCGCGAAAAGATCGGAAGAGCACACGTC1515670.6633132296037428No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGTC1502230.6574313886978239No Hit
CAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACAC1475350.6456677068859858No Hit
GAGCGTAATGGTATAAGCCTGCCTGACTGTAGATCGGAAGAGCACACGTC1297680.5679127460411469No Hit
GATCCGGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTCTG1228770.5377551822891468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTTGGGCCAACCTATGGTGTACAGATCGGAAGAGCACACGTCTGAAC1134000.4962803264369186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAATCAGATCGGAAGAGCACACG1066500.4667398308156735No Hit
AGCTCTCTGGGTCCCTACCGACGCTGGGGTGCAGATCGGAAGAGCACACG1022890.44765448245948825No Hit
CCTGAAGATTACAGGTTCGAATCCTGTCCCCGCAGATCGGAAGAGCACAC951680.416490353671505No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT938720.41081857851222603No Hit
AGTTCTTCCTATTTGACCCGAAACCGATCGATAGATCGGAAGAGCACACG909530.39804395529468306No Hit
TGATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACG869450.38050346545024594No Hit
AGGTGTGAAGCCATCTCGTACTAAACAGATCGGAAGAGCACACGTCTGAA835450.36562380839658176Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTGTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACG834940.36540061354077674No Hit
CAAGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACG776190.3396894414259893No Hit
GAGGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTC758780.3320701817405689No Hit
AGCGTAATGGTATAAGCCTGCCTGACTGTAGATCGGAAGAGCACACGTCT749790.32813582536079117Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGAGTGTTCGCCCTTGGTCTAAGATCGGAAGAGCACACGTCTGAAC734290.321352452292209Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGATATAGCTGGTTTTCCGCGAAATCAGATCGGAAGAGCACACGT728020.3186084684767244No Hit
GTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTGAA698200.3055581339667166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTG677730.2965997051464664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGTCT677380.29644653220620804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGTGCTCTCCTATCTTTTGATTAGGAAAGAGATCGGAAGAGCACACG654450.286411516434428No Hit
AGGGCTCAAGTGATTCACCGAAGCGACGAGACAGATCGGAAGAGCACACG646220.28280976415349696No Hit
GTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGA645930.28268284943156863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGAAATAGCTTAATGGTAGAGCATAGCCAGATCGGAAGAGCACACGTC641690.2808272686695823No Hit
TGGGTTGGGCCAACCTATGGTGTACCGGTTGAGATCGGAAGAGCACACGT640760.28042026628546735No Hit
GTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAAC624540.2733217945969252Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAAGATCGGAAGAGCACACGTCT596830.26119487409818887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGATATAGCTGGTTTTCCGCGAAATCTAGATCGGAAGAGCACACG595450.260590935076599No Hit
GGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTG566920.248105152260686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTCTG565350.24741806221438445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGTC558840.24456904552557993No Hit
GGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTCCA534290.23382505785889Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGT504040.2205865394508505No Hit
ACTCTAACGGCGTACCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT492340.21546618687650135Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACACGT491600.21514233551709808No Hit
TAGTTCTTCCTATTTGACCCGAAACCGATCGATAGATCGGAAGAGCACAC491140.2149410225099014No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAAAGATCGGAAGAGCACACGTCT482700.21124736646481534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTTCGATCCTTCCCAGTAAAACGCGGCAGATCGGAAGAGCACACGTCT479020.2096368624072423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTGAACT470130.20574626972468127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGGGTTGGGCCAACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGT461060.20177690238713025No Hit
AAGAAGGTTGAAACTTCCAGGAGATCGGAAGAGCACACGTCTGAACTCCA455050.199146704184409Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGTGTGAAGCCATCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAG450230.19703729397856606Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGATCGGATATAGCTGGTTTTCCGCGAAAAGATCGGAAGAGCACACGT450200.19702416486940103No Hit
AGGGAGAACTGCGAGGAGCCAACCCTAGATCGGAAGAGCACACGTCTGAA448920.1964639895450278Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGAAATAGCTTAATGGTAGAGCATAGCAGATCGGAAGAGCACACGTCT445610.19501541116715637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCCGATGGGTACACGAAAGTGACGAAGTAGATCGGAAGAGCACACGTC441720.1933130033454283No Hit
GTGATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACAC430570.18843335110577075No Hit
AGGGAGAACTGCGAGGAGCCAACCCAGATCGGAAGAGCACACGTCTGAAC423290.18524735394839797Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATGAGTGTTCGCCCTTGGTCTAAGATCGGAAGAGCACACGTCTGAA413510.18096726436060867Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTAGGTTCAAATCCTACAGAGCGAGATCGGAAGAGCACACGTCTGAACT407220.17821452780568078Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGT391480.17132612186377857Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGTTGGGCCAACCTATGGTGTACAGATCGGAAGAGCACACGTCTGAACT381530.16697163399072096Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGTCTGAAC379410.1660438436097278Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCT379230.16596506895473778Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACT372390.16297163206511828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGATGAGTGTTCGCCCTTGGTCTAGATCGGAAGAGCACACGTCTGAACTC371840.16273093173042666Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGTCTG370470.1621313690785584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAAGATTACAGGTTCGAATCCTGTCCCCGCAGATCGGAAGAGCACACG366060.16020139003130374No Hit
GAGGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTC365270.15985565682329214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTGTGAAGCCATCTCGTACTAAACGAACAGATCGGAAGAGCACACGTC364660.1595886982702705No Hit
AGCGCCGGGCAGCTAAGTTGGTATGAGATCGGAAGAGCACACGTCTGAAC363370.1590241465761756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCAGCGCCGGGCAGCTAAGTTGGTATGAGATCGGAAGAGCACACGTCTG358540.15691036000061093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCGCTTGAGAGAACCATGTCGAAGGAACAGATCGGAAGAGCACACGTC357920.15663902507786764No Hit
CGATGGCCCGAGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT351480.15382064297711479Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT345610.15125171395049686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGTC337660.14777250002177245No Hit
AGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCTGAAC336180.14712479730296588Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGTTGGGCCAACCTATGGTGTACCAGATCGGAAGAGCACACGTCTGAA332150.1453611203051345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCCGTTCTCGTTTGTACAGACAGACTTAGATCGGAAGAGCACACGTCT330900.14481407408992625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTAACGGCGTACCTTTTAGATCGGAAGAGCACACGTCTGAACTCCAG327250.1432166991415182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACGCGGCGTGTTCGAATTCTGATCGCTAGATCGGAAGAGCACACGTCT326470.14287534230322824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGATGATGGAAAAACCAGAGATCGGAAGAGCACACGTCTGAACTCCAG325030.14224514506330835Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGCCGTTCTCGTTTGTACAGACAGACTTTAGATCGGAAGAGCACACGTC324730.14211385397165838No Hit
AAAACGCGGCGTGTTCGAATTCTGATCGCTAGATCGGAAGAGCACACGTC323670.1416499587811618No Hit
AACGCGGCGTGTTCGAATTCTGATCGCTAGATCGGAAGAGCACACGTCTG321990.1409147286679219Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGAAGATTACAGGTTCGAATCCTGTCCCCGCAGATCGGAAGAGCACA312770.13687971578454589No Hit
AGGTGTGAAGCCATCTCGTACTAAACGAAAAAGATCGGAAGAGCACACGT308520.13501975865283786No Hit
CGTAGGTTCAAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAAC302670.13245958236566327Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGTGAAGCCATCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC296380.1297068458107354Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTAGATCGGAAGAGCACACGTCT291870.12773310306626406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAACTC288350.12619262092423764Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGTCTGAACTCCAGT282060.12343988436930976Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTTGTTAGATCGGAAGAGCACAC281720.12329108779877311No Hit
GGGTTGGGCCAACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGTCT278180.12174185291730338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGTAGCGCAGTCTGGTCAGCGCATCTGAGATCGGAAGAGCACACGTC274790.12025826358165863No Hit
AAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCTGAACTCC271050.11862150130575555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAAGACACGGGTTCGATTCCCGTAAGGGAGATCGGAAGAGCACACGTCT269910.11812259515748563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTTGGGCCAACCTATGGTGTACCGGTTGAGATCGGAAGAGCACACG269160.11779436742836069No Hit
AATGGTATAAGCCTGCCTGACTGTAGATCGGAAGAGCACACGTCTGAACT268290.11741362326257575Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTGACCCGAAACCGATCGATAGATCGGAAGAGCACACGTCTGAACTCC264180.11561493530697103Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCCGGGAGTCCCGTGTGGAAGGGCTCTCGCAGATCGGAAGAGCACACG263350.11525169662007276No Hit
AGGTGTGAAGCCATCTCGTACTAAACGAAGATCGGAAGAGCACACGTCTG262390.11483156512679285Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCACTCACTCTAACGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG257860.11284906964287816Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCGACTCATCACATCCTGGGGTAGATCGGAAGAGCACACGTCTGAACT257040.11249020732570156Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAACT256480.11224513062128827Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGATATAGCTGGTTTTCCGCGAAATCTAAGATCGGAAGAGCACAC255830.11196066658937998No Hit
GGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTCTGAACTC255150.1116630734483067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTCTTCCTATTTGACCCGAAACCGATCGAAGATCGGAAGAGCACACGT251340.10999567658435197No Hit
GCACTCACTCTAACGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT249800.1093217156472154Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGTCT249250.10908101531252377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTGTGAAGCCATCTCGTACTAAACGAAAAAGATCGGAAGAGCACACG247570.10834578519928391No Hit
TGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAA246110.10770683521992067Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCTGAA243520.10657335546200919Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTAGGTTCAAATCCTACTTGGGGAGAGATCGGAAGAGCACACGTCTGAA241330.10561493049296436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAATCTAGATCGGAAGAGCACAC241110.1055186503590877No Hit
AACCGGGCACTACGGTGAGACGTGAAAACACCCAGATCGGAAGAGCACAC238180.10423637403063958No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAAGATCGGAAGAGCACACGTCTG232270.101649939525135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTTGTAGATCGGAAGAGCACACG231310.10122980803185506No Hit
TAGCAGCGCCGGGCAGCTAAGTTGGTATGAGATCGGAAGAGCACACGTCT231200.10118166796491675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGTCT228640.10006131731617027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)